FastQCFastQC Report
Tue 27 Jun 2023
SRR1027794.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1027794.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47214913
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAATCTTTGAACGCACATTGCGCCCTCTGGCTGTAGGC31268596.622608835475351No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGC21469964.547283609312168No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAG15213713.222225570975848No Hit
TTTTACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCA10785472.2843354598577785No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA9245361.958144029620472Illumina Single End Adapter 1 (95% over 22bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGG8836381.8715230927143718No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCTGCTGTAGGCA8519771.804465889834426No Hit
CGAATCTTTGAACGCACATTGCGCCCTCTGGCTGTAGGCA7997631.693877949113239No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCTCTGTAGGCAC7586061.606708456711548No Hit
TTTTACTTATTCAATGAAGCGGAGCTGGAGCTGTAGGCAC7542211.5974211368344573No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAG5268961.1159524957718336No Hit
TCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGCA4667290.9885203007786968No Hit
TCAACTGTATCTAATATCTGAACAAGTTAACCTGTAGGCA4501580.9534233389353063No Hit
TTTTTACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGC4420250.9361978491837949No Hit
TTTTTACTTATTCAATGAAGCGGAGCTGGAGCTGTAGGCA4292160.9090687088632357No Hit
TTCGAATCTTTGAACGCACATTGCGCCCTCTGGCTGTAGG4272710.9049492477090871No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCTGGTCTGTAGG3863910.8183664343509433No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCCTGTAGGCACC3644580.771912891166399No Hit
TTTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGG3111550.6590184757938662No Hit
CGAATCTTTGAACGCACATTGCGCCCTCTGGTCTGTAGGC3054770.6469926143885937No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGG2802100.5934777429326197No Hit
ATCGAATCTTTGAACGCACATTGCGCCCTCTCTGTAGGCA2754870.5834745475439085No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGG2737550.5798062150405742No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGG2673350.5662088162695544No Hit
AGTTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACC2661140.5636227689331971No Hit
TTTACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCAC2633900.5578534053425027No Hit
CCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGCAC2499080.5292988679233614No Hit
TTGGGTTTGGTGTTGAGCAATACGACTCTGTAGGCACCAT2338620.4953138428953581No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGC2304800.4881508518293786No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCA2113780.44769329554837894No Hit
TTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGC2041720.4324311685166083No Hit
ATCGAATCTTTGAACGCACATTGCGCCCTCTGCTGTAGGC1947920.41256456408169173No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGCGCTGTAGGCA1895120.401381656681227No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA1845780.3909315685914745No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCA1780480.3771011925829451No Hit
TTTACTTATTCAATGAAGCGGAGCTGGAGCTGTAGGCACC1729090.3662169196414701No Hit
ATTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAG1728660.3661258467213526No Hit
TAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATC1523480.3226692380011375No Hit
TTCGAATCTTTGAACGCACATTGCGCCCTCTGCTGTAGGC1379520.29217887153577937No Hit
CGAATCTTTGAACGCACATTGCGCCCTCTGCTGTAGGCAC1353060.2865747099862283No Hit
AGCGGAGGAAAAGAAACCAACAGGGATTGCCTCTGTAGGC1350350.2860007387919999No Hit
ATCGAATCTTTGAACGCACATTGCGCCCTCCTGTAGGCAC1345130.2848951559012721No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGC1300250.2753896846108771No Hit
TTCGAATCTTTGAACGCACATTGCGCCCTCTCTGTAGGCA1250750.26490570892294135No Hit
ATCGAATCTTTGAACGCACATTGCGCCCTCTGGCTGTAGG1244710.2636264520915246No Hit
ATTTTAGATGGATACGAATAAGACTTTCTGTAGGCACCAT1225640.25958747398306126No Hit
TTTCCGGAGGGCATGCCTGTTTGAGCGTCGTTCTGTAGGC1102060.2334135403362916No Hit
CGAATCTTTGAACGCACATTGCGCCCTCTCTGTAGGCACC1094930.23190342424225158No Hit
ATCAACTGTATCTAATATCTGAACAAGTTAACCTGTAGGC1091230.2311197735342645No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCA992240.2101539401332795No Hit
GAATCTTTGAACGCACATTGCGCCCTCTGGCTGTAGGCAC980890.20775003863715688No Hit
AACTGTATCTAATATCTGAACAAGTTAACCTGTAGGCACC909840.19270182706891784No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCTGTAGGCACCA900330.1906876329519023No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGC855370.18116521786241563No Hit
CGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGCACC850220.18007446079589304No Hit
TTTTTTACTTATTCAATGAAGCGGAGCTGGAGCTGTAGGC810960.1717592913916838No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA702200.14872419652663557Illumina Single End Adapter 1 (95% over 22bp)
ACGGGGAGGTAGTGACAATAAATAACGACTGTAGGCACCA684760.14503044832466386No Hit
TTCGAATCTTTGAACGCACATTGCGCCCTCCTGTAGGCAC663340.1404937461178844No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA659050.13958513489159666Illumina Single End Adapter 1 (95% over 22bp)
ATTCCGGAGGGCATGCCTGTTTGAGCGTCGTTCTGTAGGC599590.1269916562167551No Hit
TTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACC587050.12433571570914469No Hit
TTTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCAC576810.1221669094254182No Hit
TTAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCAT558700.11833125690605423No Hit
ACCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCAC544300.11528137306956385No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACC525060.11120638938803085No Hit
TACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCACCA517430.10959037454966825No Hit
ATCCCGACTGTTTGGAAGGGATGTATTTATTAGATCTGTA515090.10909476842623855No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCA505120.10698314746444625No Hit
GGAATGAGTACAATGTAAATACCTTAACGACTGTAGGCAC492210.10424884188603716No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCA488030.10336352838350037No Hit
AGCGGAGGAAAAGAAACCAACAGGGATTGCCCTGTAGGCA486100.1029547592304152No Hit
GGCGCCGGTGAAATACCACTACCTCTATCTGTAGGCACCA485110.10274507971665649No Hit
TCCGGAGGGCATGCCTGTTTGAGCGTCGTTCTGTAGGCAC477060.10104010993306288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)