FastQCFastQC Report
Thu 29 Jun 2023
SRR1027795.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1027795.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86377579
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATT62811367.271720361599855No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA37531514.34505231965346No Hit
TTTTACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCACCATCAATTC35306264.087433383609883No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCTGGCTGTAGGCACCATCAATT28653873.317280980982345No Hit
TTTTACTTATTCAATGAAGCGGAGCTGGAGCTGTAGGCACCATCAATTCG25311372.930317137043167No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT23808692.7563506960527335No Hit
TTTTTACTTATTCAATGAAGCGGAGCTGGAGCTGTAGGCACCATCAATTC14038691.6252701409934167No Hit
TTTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGCACCATCAAT13945281.6144559921041548No Hit
TTTTTACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCACCATCAATT13939881.6138308298731086No Hit
TCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGCACCATCAATTC13776391.5949034644742706No Hit
TTTACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCACCATCAATTCG10847751.2558525170055992No Hit
AGTTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT9666391.119085544178079No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCTGCTGTAGGCACCATCAATTC9217571.0671253011154667No Hit
TCAACTGTATCTAATATCTGAACAAGTTAACCTGTAGGCACCATCAATTC8644801.000815269434676No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCTCTGTAGGCACCATCAATTCG8577320.9930030569622703No Hit
CCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGCACCATCAATTCG7506340.8690148632204661No Hit
TTTACTTATTCAATGAAGCGGAGCTGGAGCTGTAGGCACCATCAATTCGT7346940.8505610003262536No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT7029770.813841980914978No Hit
TAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG6375850.7381371501509668No Hit
TTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGCACCATCAATT6066750.7023524009627545No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATTC5716740.6618314690204503No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATTC5417690.6272102162066848No Hit
CGAATCTTTGAACGCACATTGCGCCCTCTGGCTGTAGGCACCATCAATTC5399400.625092768575975No Hit
TTTCCGGAGGGCATGCCTGTTTGAGCGTCGTTCTGTAGGCACCATCAATT5180030.5996961317936452No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA4864690.563188972916224No Hit
TTGGGTTTGGTGTTGAGCAATACGACTCTGTAGGCACCATCAATTCGTAT4587220.5310660536109724No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCCTGTAGGCACCATCAATTCGT4506400.5217094588863159No Hit
ATTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA4018820.4652619402541949No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3815900.4417697328608851No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCTGGTCTGTAGGCACCATCAAT3622980.41943523330284593No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGCGCTGTAGGCACCATCAATTC3480930.4029899935028279No Hit
TTCGAATCTTTGAACGCACATTGCGCCCTCTGGCTGTAGGCACCATCAAT3198730.37031947839149326No Hit
TTTTTTACTTATTCAATGAAGCGGAGCTGGAGCTGTAGGCACCATCAATT2782190.3220963162211342No Hit
CGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGCACCATCAATTCGT2506950.29023156576314785No Hit
AGCGGAGGAAAAGAAACCAACAGGGATTGCCTCTGTAGGCACCATCAATT2464990.28537382368635267No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATT2417150.27983534940241844No Hit
ATTTTAGATGGATACGAATAAGACTTTCTGTAGGCACCATCAATTCGTAT2386580.2762962365499964No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATTC2309600.2673842016340838No Hit
TTAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT2202120.254941157820596No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT2177530.2520943542536657No Hit
ATCGAATCTTTGAACGCACATTGCGCCCTCTCTGTAGGCACCATCAATTC2126890.24623172177585576No Hit
CGAATCTTTGAACGCACATTGCGCCCTCTGGTCTGTAGGCACCATCAATT2057100.23815207879350264No Hit
ATTCCGGAGGGCATGCCTGTTTGAGCGTCGTTCTGTAGGCACCATCAATT2053050.2376832071202181No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1957860.2266629862362778No Hit
TACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCACCATCAATTCGTA1907090.2207853035566093No Hit
ATCAACTGTATCTAATATCTGAACAAGTTAACCTGTAGGCACCATCAATT1889150.21870837570013396No Hit
ACCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATTCG1844630.21355426041750952No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATT1657840.1919294357624911No Hit
AACTGTATCTAATATCTGAACAAGTTAACCTGTAGGCACCATCAATTCGT1561470.1807726053539889No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1513580.1752283425308783Illumina Single End Adapter 1 (95% over 22bp)
TCCGGAGGGCATGCCTGTTTGAGCGTCGTTCTGTAGGCACCATCAATTCG1409050.16312682252879535No Hit
ATCGAATCTTTGAACGCACATTGCGCCCTCTGCTGTAGGCACCATCAATT1398920.1619540644916663No Hit
TTTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATTCG1392610.16122355084761059No Hit
ACGGGGAGGTAGTGACAATAAATAACGACTGTAGGCACCATCAATTCGTA1349870.1562755075596643No Hit
TTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATTCGT1285180.14878629557329917No Hit
TACTTATTCAATGAAGCGGAGCTGGAGCTGTAGGCACCATCAATTCGTAT1259420.14580404018964227No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATTCGT1218680.14108753846875008No Hit
TCGAATCTTTGAACGCACATTGCGCCCTCTGTAGGCACCATCAATTCGTA1171000.13556758751018017No Hit
GTTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGTA1166290.13502230711976773No Hit
TTCGAATCTTTGAACGCACATTGCGCCCTCTGCTGTAGGCACCATCAATT1132090.1310629463231425No Hit
CTTACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCACCATCAATTCG1123080.13001985156356372No Hit
ACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCACCATCAATTCGTAT1109850.1284882040975008No Hit
TAGGGTGCACCATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC1094600.12672269964871324No Hit
TTCGAATCTTTGAACGCACATTGCGCCCTCTCTGTAGGCACCATCAATTC1092740.12650736599135293No Hit
TTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATTCG1063100.12307591996761104No Hit
AAATGATTAATAGGGACGGTCGGGGGTACTGTAGGCACCATCAATTCGTA1062850.12304697727172928No Hit
ATCGAATCTTTGAACGCACATTGCGCCCTCCTGTAGGCACCATCAATTCG1062470.12300298437398899No Hit
ATTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTGTAGGCACCATCAAT1059170.12262094078834972No Hit
ATCCCGACTGTTTGGAAGGGATGTATTTATTAGATCTGTAGGCACCATCA1049030.12144702504338538No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATT1035860.1199223238243341No Hit
CGAATCTTTGAACGCACATTGCGCCCTCTGCTGTAGGCACCATCAATTCG1020310.1181220881404884No Hit
TTTTTTACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCACCATCAAT1012390.11720518353495414No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1009430.11686250201571405No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATTCG998570.11560523130661024No Hit
GGCGCCGGTGAAATACCACTACCTCTATCTGTAGGCACCATCAATTCGTA975570.11294250328548802No Hit
AGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATGC940560.10888936815420586No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATTC908090.10513029081308241No Hit
TTACTTATTCAATGAAGCGGAGCTGGAGGCTGTAGGCACCATCAATTCGT893250.10341225238554093No Hit
AGCGGAGGAAAAGAAACCAACAGGGATTGCCCTGTAGGCACCATCAATTC881770.10208320379065033No Hit
CGAATCTTTGAACGCACATTGCGCCCTCTCTGTAGGCACCATCAATTCGT866710.10033969579073292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)