FastQCFastQC Report
Tue 18 Jul 2023
SRR10294592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10294592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24325319
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length67
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4598311.8903390331695136Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGAGGTTGTCCCAGGTTCGAGTCCTGGCGGTGGCGCCTGTAGGCACCAT4057061.66783424299595No Hit
ATCAGCCGAGGGTTAGTCGATCCTAAGTCTAACCGCTGTAGGCACCATCA3273861.3458651868039222No Hit
TGTAGTGGCCCATCATACGACCCTGCTGTAGGCACCATCAATAGATCGGA2704101.1116400981216321No Hit
ACGAGGTTGTCCCAGGTTCGAGTCCTGGCGGTGGCGCCCTGTAGGCACCA1793440.737272962381295No Hit
ACGGTGATGACCAGGGTTCGAATCCCTGACGGAGCCTGTAGGCACCATCA1756700.7221693577790285No Hit
TCAGCCGAGGGTTAGTCGATCCTAAGTCTAACCGCTGTAGGCACCATCAA1614680.6637857452146876No Hit
ACACTGCACGCAAGTGCGATAAGGGGACCCCAAGTGCGAGGGCCTGTAGG1524100.626548823470722No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA1471640.604982816463784No Hit
ACGAGGTTGTCCCAGGTTCGAGTCCTGGCGGTGGCGCTGTAGGCACCATC1462080.6010527549505106No Hit
ACAGAGTCGTCACCGGCAAGAGCCTGTAGGCACCATCAATAGATCGGAAG1433930.5894804503899825No Hit
ATGTACACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1412340.5806049244410731Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCCCCGTCGAATCACCGAAATTCGTGGAAGCCGTCTGTAGGCACCATC1234960.507685017409227No Hit
AATCCCGCCTCGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1201420.4938969145687257No Hit
ACACTGCACGCAAGTGCGATAAGGGGACCCCAAGTGCGAGGGCACTGTAG1169790.480894001842278No Hit
ATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAA1144990.4706988631885979No Hit
AATCTCCGTCAGGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1075060.4419510387510232No Hit
AATCCCGCCTCGGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1048260.4309337114962398No Hit
ATGCCGGGCAGCTACGCACCACGGGGTAAGAGCTGCTGTAGGCACCATCA973220.4000851951828463No Hit
AATCTCCGTCAGGACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA954140.39224151592832146No Hit
ACGGTGATGACCAGGGTTCGAATCCCTGACGGAGCACCTGTAGGCACCAT803430.330285493892187No Hit
ATCCTCTGGGAAATCCAGTTCGCCGCCCCTCTGTAGGCACCATCAATAGA782380.3216319588655754No Hit
TTAAGGCGGCCAGAGCGGTGAGGCTGTAGGCACCATCAATAGATCGGAAG739570.3040330118589606No Hit
AATCCCTCCACCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG725000.29804336789992353No Hit
ACTATGACCCTCTTAAGGTAGCGTAGTACCTTGCCGCTCTGTAGGCACCA638460.2624672671301865No Hit
TCAATCAGCCGAGGGTTAGTCGATCCTAAGTCTAACCGCTGTAGGCACCA636290.26157519249798944No Hit
ACGATGCTCGCTAGGTGTGACACAGGCTCTGTAGGCACCATCAATAGATC618870.25441392978238025No Hit
ACGCCGGACATCTCACCAGCTCCGACTACAGTGCTGTAGGCACCATCAAT591530.243174611605299No Hit
AAGCAGGCAAGGGTGAGGTTGTTCGCCCTGTAGGCACCATCAATAGATCG589730.24243464186430608No Hit
AATCTCCGTCAGGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC588540.24194543964664966No Hit
ACGGTGATGACCAGGGTTCGAATCCCTGACGGAGCACCCTGTAGGCACCA582000.23925688292104208No Hit
TCGGTAGGACCTGAGTTCGACTCTCAGCGGTCCCACCTGTAGGCACCATC571280.23484995201912873No Hit
ATCTCCTAAACTCGATCGTAGTTCGGATTGAGGGCTGCTGTAGGCACCAT537690.22104129446359985No Hit
AATCTGCCCCAACCCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC536600.22059320167599855No Hit
AGCGATCCTCTGGGAAATCCAGTTCGCCGCCCCTCTGTAGGCACCATCAA532840.2190474871059245No Hit
AATCCCGCCTCGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC510260.20976497779946895No Hit
ACGAGGTTGTCCCAGGTTCGAGTCCTGGCGGTGGCGCCACTGTAGGCACC498670.20500039485607569No Hit
ACGCCGGACATCTCACCAGCTCCGACTACAGTGATGCTGTAGGCACCATC491100.2018884110008999No Hit
ACGAGAGCGTCACTGTCCCAACGTTGGGCCCGGTGAACAGCTGTAGGCAC488030.20062635149820646No Hit
ATCCTGCCGGAGGTCATTGCTATTGGGGTCCGCTGTAGGCACCATCAATA486810.20012481645153346No Hit
TGGACTAGGTCGGGCAGTTAGGCCCTGCTCTTCTGTAGGCACCATCAATA471920.1940036223163199No Hit
GCCGCCGTAGCTCAGTTGGTAGAGCACCTCGCCTGTAGGCACCATCAATA470060.19323898691729388No Hit
ATGGGACCCGTAGATGACAGAAAAGCTACCTTAGGGATCTGTAGGCACCA462430.190102337404085No Hit
AATCTGCCCCAACCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACA455670.1873233399323561No Hit
ACTGCACGCAAGTGCGATAAGGGGACCCCAAGTGCGAGGGCACTGTAGGC454250.18673958602557278No Hit
AACAGAGTCGTCACCGGCAAGAGCCTGTAGGCACCATCAATAGATCGGAA450330.18512809636741043No Hit
TCCCCACGTCGGAACGCCGGGCAACCTCGCTGTCCACCCCTGTAGGCACC435040.1788424645119762No Hit
AGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAAGA431690.17746529860512825No Hit
ACAGAGTCGTCACCGGCAAGAGCTGTAGGCACCATCAATAGATCGGAAGA429370.1765115598278485No Hit
ATCGCTGCCATTCACCCGCCAACACCCCGCGACCAGGCTGTAGGCACCAT425260.17482196225258134No Hit
ACTGCACGCAAGTGCGATAAGGGGACCCCAAGTGCGAGGGCCTGTAGGCA420370.17281171112288393No Hit
AGCGGGATGGGATAGCCAGGAGATTCCGCCGGGCCTGTAGGCACCATCAA408230.1678210263141873No Hit
ATGCTCGCTAGGTGTGACACAGGCTCTGTAGGCACCATCAATAGATCGGA401580.165087249215519No Hit
AGGCGGCCAGAGCGGTGAGGCTGTAGGCACCATCAATAGATCGGAAGAGC400810.16477070660409426No Hit
AAGCAGGCAAGGGTGAGGTTGTTCGCCTCTGTAGGCACCATCAATAGATC393400.1617244978370068No Hit
TTCCACGCGGGCCACGGCCGGCCGACCTACCCCGTCCTGTAGGCACCATC390570.1605611009664457No Hit
TCGGTAGGACCTGAGTTCGACTCTCAGCGGTCCCACCCTGTAGGCACCAT380980.1566187066241557No Hit
AATCCGGTCCCACGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC375650.15442757400221555No Hit
ACAATGGGACGAAGGACCCGTCAACGGCGGGGGTCTGTAGGCACCATCAA375170.15423024873795077No Hit
AATCAGCCGAGGGTTAGTCGATCCTAAGTCTAACCGCTGTAGGCACCATC366340.15060028606407996No Hit
ACCCGCCAACACCCCGCGACCAGGCTGTAGGCACCATCAATAGATCGGAA357720.14705665319332503No Hit
ATAGTCCTCGCTTTTCTCGACCGTACTGTAGGCACCATCAATAGATCGGA356620.14660444946271825No Hit
ATCAGCCGAGGGTTAGTCGATCCTAAGCTGTAGGCACCATCAATAGATCG353090.14515328658177104No Hit
ATCAGCCGAGGGTTAGTCGATCCTAAGTCTCTGTAGGCACCATCAATAGA350280.14399811159722098No Hit
ACTATGACCCTCTTAAGGTAGCGTAGTACCTTGCCCTGTAGGCACCATCA349810.1438048972759617No Hit
TCCACGCGGGCCACGGCCGGCCGACCTACCCCGTCCTGTAGGCACCATCA340570.14000638593886477No Hit
ATCGGGACTCAAATCCACCTCCGAGACCTGGCGGCGTGTCCTGTAGGCAC334270.13741649184538957No Hit
ACAGGGCGGTGCAATAGGGCTTGAAATCAGTTGGCGCTGTAGGCACCATC327190.1345059441974841No Hit
AAGCTTGTGCACCGTGAGGGAGCTGTAGGCACCATCAATAGATCGGAAGA324050.13321510809375203No Hit
TCGGTTCCCTCCATCCTGCCTGTGCCTGTAGGCACCATCAATAGATCGGA318980.13113085998995533No Hit
ACGAGAGCGTCACTGTCCCAACGTTGGGCCCGGTGCTGTAGGCACCATCA315260.1296015891919033No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC313050.12869307078768422Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATCTCACCCTCGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA310020.12744745505701283No Hit
ACGAGTTGTTAAGCCCGCGAGCACTAACAGTCCAAGCCCTGTAGGCACCA303570.12479589681845488No Hit
TTGTAGTGGCCCATCATACGACCCTGCTGTAGGCACCATCAATAGATCGG299780.12323784941936425No Hit
ATCGACCCGGTGGCTTGCTACCTCGCTGTAGGCACCATCAATAGATCGGA296830.12202512123273698No Hit
GCCGCCGTAGCTCAGTTGGTAGAGCACCTCGCTGCTGTAGGCACCATCAA294450.12104671679742411No Hit
ACGGACCTGGGTCTTATGTGCTGTAGGCACCATCAATAGATCGGAAGAGC294410.12103027302540206No Hit
AATCCGGTCCCACGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACA290090.11925434564701906No Hit
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT285810.11749486204065812Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGGACCCGTAGATGACAGAAAAGCTACCTTAGGGACTGTAGGCACCAT278280.11439932195750444No Hit
ACACCTTCAGCAAGCGGACGCTGTAGGCACCATCAATAGATCGGAAGAGC271710.1116984324028803No Hit
ATGTCGGTTCCCTCCATCCTGCCTGTGCCTGTAGGCACCATCAATAGATC268380.11032948838204343No Hit
TGTAGTGGCCCATCATACGACCCTCTGTAGGCACCATCAATAGATCGGAA264040.10854533911764938No Hit
ACGGTGATGACCAGGGTTCGAATCCCTGACGGAGCACTGTAGGCACCATC263290.10823701839223568No Hit
AATCTCACCCTCGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC257020.10565945712777702No Hit
ACAACCGGAGGAGCCTGCGGCTGTAGGCACCATCAATAGATCGGAAGAGC249940.10274890947987157No Hit
TGTAGTCCGGCCAATCATATCACCCTCCTGTAGGCACCATCAATAGATCG249410.10253102950057921No Hit
ACACATCTTCAGCAAGCAGACGCTGTAGGCACCATCAATAGATCGGAAGA245580.10095653832946651No Hit
TCACCCGCCAACACCCCGCGACCAGGCTGTAGGCACCATCAATAGATCGG244280.10042211573874942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)