FastQCFastQC Report
Tue 18 Jul 2023
SRR10294593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10294593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108269604
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6121950.5654357062209261Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCGATCCTCTGGGAAATCCAGTTCGCCGCCCCTCTGTAGGCACCATCAA5877000.5428116279062035No Hit
ATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAA3556310.3284679973522393No Hit
ATCAGCCGAGGGTTAGTCGATCCTAAGTCTAACCGCTGTAGGCACCATCA2822230.2606668811682363No Hit
ATCCTCTGGGAAATCCAGTTCGCCGCCCCTCTGTAGGCACCATCAATAGA2192910.20254161084767613No Hit
ATAGTCCTCGCTTTTCTCGACCGTACTGTAGGCACCATCAATAGATCGGA1918770.1772214849885292No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA1910330.17644194948750344No Hit
ATGTACACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1892630.17480714162397787Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAAGA1847280.17061852373635725No Hit
ACAGAGTCGTCACCGGCAAGAGCCTGTAGGCACCATCAATAGATCGGAAG1827040.16874911632631445No Hit
GCAATGGCGAAAGGTAAGGTCGCTGTAGGCACCATCAATAGATCGGAAGA1816820.16780517641867426No Hit
TTAAGGCGGCCAGAGCGGTGAGGCTGTAGGCACCATCAATAGATCGGAAG1735430.16028783110724223No Hit
TGTAGTGGCCCATCATACGACCCTGCTGTAGGCACCATCAATAGATCGGA1597320.14753171167043339No Hit
ATTCCTTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1524400.14079667272081275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACAGAGTCGTCACCGGCAAGAGCCTGTAGGCACCATCAATAGATCGGAA1488920.13751966803166657No Hit
AAGGTCGATATCTATGCAGATGCCACGCCGCTGTAGGCACCATCAATAGA1410390.13026647811513192No Hit
AATCCGGTCCCACGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1401140.12941212937289398No Hit
ATTTCCGGCACGGCTTGCTTTCCTTCTCTGTAGGCACCATCAATAGATCG1391210.12849497445284827No Hit
AATCGCGTCCGGCCCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1316450.12158998937504195No Hit
AATCCGGTCCCACGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCA1293270.11944903760800678No Hit
TCAGCCGAGGGTTAGTCGATCCTAAGTCTAACCGCTGTAGGCACCATCAA1248760.11533800382238397No Hit
GTCAGCGGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1220590.11273616554467124No Hit
ACTATGACCCTCTTAAGGTAGCGTAGTACCTTGCCCTGTAGGCACCATCA1148160.10604638398788269No Hit
GTATGGCAGACCTCATTGTCAAGGCAGCCGCTGTAGGCACCATCAATAGA1130530.10441804146619027No Hit
ATGAGCGACCTCGAAATCGCTGTAGGCACCATCAATAGATCGGAAGAGCA1130460.10441157612435711No Hit
AATCCCGCCTCGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA1126300.10402735009541551No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)