FastQCFastQC Report
Tue 18 Jul 2023
SRR10294594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10294594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4480382
Total Bases448 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGA604981.35028664966514No Hit
ATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAA514491.1483172640190056No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA393270.877759976716271Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT282820.6312408183052249No Hit
TATAGTCCTCGCTTTTCTCGACCGCTGTAGGCACCATCAATAGATCGGAA237750.5306467171772407No Hit
TAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAAG228130.5091753337103845No Hit
TAACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC192390.4294053498116902No Hit
ATAGTCCTCGCTTTTCTCGACCGCTGTAGGCACCATCAATAGATCGGAAG182150.40655015576796805No Hit
ACGCCGGACATCTCACCAGCTCCGCTGTAGGCACCATCAATAGATCGGAA168800.37675358931448255No Hit
ACGCTTTAGGAAACACCGAGCGGGGCCTGTAGGCACCATCAATAGATCGG150560.33604277492410245No Hit
ACGCCGGACATCTCACCAGCTCCGACTACAGTGCTGTAGGCACCATCAAT133740.2985013331452542No Hit
AAACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC122390.2731686717784332No Hit
ATGCCGGGCAGCTACGCACCACGGGGTAAGAGCTGCTGTAGGCACCATCA97770.21821800016159337No Hit
TAGGGAAACTGGTTAATATTCCAGTACCTTCTTGCCTGTAGGCACCATCA83130.18554221492720935No Hit
AACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG80470.17960522116194558No Hit
ATATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGG75520.16855705607245097No Hit
ACGAGAGCGTCACTGTCCCAACGTTGGGCCCGGTGCTGTAGGCACCATCA72290.16134784935748783No Hit
TAGTCCTCGCTTTTCTCGACCGCTGTAGGCACCATCAATAGATCGGAAGA69170.15438415742229122No Hit
TAGGCGGCCAGAGCGGTGAGGCTGTAGGCACCATCAATAGATCGGAAGAG67140.14985329375932677No Hit
ATGGGACCCGTAGATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC59910.13371627687103466No Hit
ACGAGTGTCGCTGACGCGAAAGCGTGGCTGTAGGCACCATCAATAGATCG59380.13253334202306857No Hit
ACGCTTTAGGAAACACCGAGCGGGGCCCTGTAGGCACCATCAATAGATCG59030.13175215863290227No Hit
ACAGCCGGGAGGTGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC54490.1216190940861739No Hit
ACGGAAGTCTGACAGTGACATTCCTGTAGGCACCATCAATAGATCGGAAG54360.12132894025554072No Hit
ACGACGTGTTCTTCCACATGGAAGATGCTGTAGGCACCATCAATAGATCG53780.12003440778040801No Hit
AGCAAGAAGCTCGTACCGAGCTGTAGGCACCATCAATAGATCGGAAGAGC51760.11552586364287688No Hit
ACGGACCTGGGTCTTATGTGCTGTAGGCACCATCAATAGATCGGAAGAGC48250.10769171021578071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)