FastQCFastQC Report
Tue 18 Jul 2023
SRR10294595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10294595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8990526
Total Bases449.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8655139.626945075293705Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAA1423681.5835335997026203No Hit
CTGTCGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA895910.996504542670807Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAGTCCTCGCTTTTCTCGACCGCTGTAGGCACCATCAATAGATCGGAAG728480.8102751718864947No Hit
ACGCTTTAGGAAACACCGAGCGGGGCCTGTAGGCACCATCAATAGATCGG537420.5977625780738525No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC475430.5288122185509502Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCGGACATCTCACCAGCTCCGCTGTAGGCACCATCAATAGATCGGAA454670.5057212447859002No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA319980.3559079858063922Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCCGGACATCTCACCAGCTCCGACTACAGTGCTGTAGGCACCATCAAT298780.3323276079731041No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC274110.30488761169257506Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCCAAGGCCTGTCGGGATCAACCGACGTTAGGGCTGTAGGCACCATCA258170.28715783703867825No Hit
AAGCATGACCTCTGATGAGATAGTTCGTGAGGCTGTAGGCACCATCAATA200550.22306814973895853No Hit
AGCCAAGGCCTGTCGGGATCAACCGACGTTAGGGCTGTAGGCACCATCAA179800.19998829879364122No Hit
CTGTAGGCACCATCAATATATCGGAAGAGCACACGTCTGAACTCCAGTCA174270.1938373794814675Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACAGCCGGGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT157000.17462826980312388Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAGTCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA156250.17379405832317266Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAAG149770.16658647113639402No Hit
ATGCTCGCTAGGTGTGACACAGGCCTGTAGGCACCATCAATAGATCGGAA144910.16118078074631007No Hit
AACTTGGCAACAGCTGGTTCCAACCGAAACATGCTGTAGGCACCATCAAT132450.147321747359387No Hit
ATCCTCTGGGAAATCCAGTTCGCCGCCCCTCTGTAGGCACCATCAATAGA120240.13374078446578097No Hit
ATGCCGGGCAGCTACGCACCACGGGGTAAGAGCTGCTGTAGGCACCATCA120140.13362955626845416No Hit
ACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT119960.13342934551326585No Hit
AACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG116830.12994790293693606No Hit
AGCAAGCCAAGGCCTGTCGGGATCAACCGACGTTAGGGCTGTAGGCACCA101230.11259630415395051No Hit
CTGTAGGCACCATCAATAGATCGTAAGAGCACACGTCTGAACTCCAGTCA98300.10933731797227438Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATGTACACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA94680.10531085722904311Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA91550.10182941465271332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)