FastQCFastQC Report
Tue 18 Jul 2023
SRR10294596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10294596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9421109
Total Bases471 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA427940345.423558946192Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTCGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4344294.6112299518029145Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1621191.72080590512221Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1531681.6257958590649997Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1240481.316702736376365Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGCACCATCAATATATCGGAAGAGCACACGTCTGAACTCCAGTCA869230.9226408483332482Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTCGGCACCATCAATATATCGGAAGAGCACACGTCTGAACTCCAGTCA853700.9061565894206298Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGTCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA769360.8166342200265383Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAA560760.5952165504082375No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG486140.5160114377192748TruSeq Adapter, Index 13 (100% over 35bp)
CTGTAGGCACCATCAATAGATCGTAAGAGCACACGTCTGAACTCCAGTCA464160.4926808510547962Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGACGAGCACACGTCTGAACTCCAGTCA367560.3901451517013549Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGCTCGGAAGAGCACACGTCTGAACTCCAGTCA357490.37945638883914834Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTATGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA331670.35204984890844593Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTCGGCACCATCAATAGATCGTAAGAGCACACGTCTGAACTCCAGTCA254140.2697559278849231Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTGTCGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA248520.2637906004484185Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAGTCCTCGCTTTTCTCGACCGCTGTAGGCACCATCAATAGATCGGAAG247400.26260178074576995No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA211990.2250159721111389Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGG184070.19538039523797038TruSeq Adapter, Index 15 (97% over 36bp)
CTGTCTGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA172300.18288717389852935Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCTTTAGGAAACACCGAGCGGGGCCTGTAGGCACCATCAATAGATCGG169230.17962853417787653No Hit
CTGTAGGCACCATCAATAGATCGAAGAGCACACGTCTGAACTCCAGTCAC152540.16191299771608628Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
CTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA147880.15696665859613768Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA146870.15589459797142777Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATAGGAAGAGCACACGTCTGAACTCCAGTCA130020.1380092301235449Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTTA129610.13757403719668246TruSeq Adapter, Index 14 (97% over 37bp)
CTGTCGGCACCATCAATAGATCGGACGAGCACACGTCTGAACTCCAGTCA125410.13311596331175024Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC123260.13083385406113016Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTGGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA105010.11146246158493654Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACTTCTGAACTCCATTCA103670.11004012372641055Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CTGTAGGCACCATCAATAGATCGGAATAGCACACGTCTGAACTCCAGTCA96310.10222787996614836Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC96050.1019519039637478Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)