FastQCFastQC Report
Tue 25 Jul 2023
SRR10294597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10294597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16070885
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2421221.5065878450377812Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1930411.2011846267333754No Hit
TATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGA1644211.0230986034683218No Hit
ATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAA1384350.8614024678790247No Hit
TAACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1336570.831671684540086No Hit
ACGCCGGACATCTCACCAGCTCCGCTGTAGGCACCATCAATAGATCGGAA1028730.6401203169582758No Hit
TATAGTCCTCGCTTTTCTCGACCGCTGTAGGCACCATCAATAGATCGGAA906830.5642688626046418No Hit
TAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAAG873670.5436352758419962No Hit
ACAGCCGGGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT854330.5316010910413459Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGTCCTCGCTTTTCTCGACCGCTGTAGGCACCATCAATAGATCGGAAG791660.49260510544378855No Hit
AAACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC787030.48972411911353975No Hit
ACGCCGGACATCTCACCAGCTCCGACTACAGTGCTGTAGGCACCATCAAT738650.45961998981387775No Hit
ATGCCGGGCAGCTACGCACCACGGGGTAAGAGCTGCTGTAGGCACCATCA732180.45559407587074385No Hit
ACGCTTTAGGAAACACCGAGCGGGGCCTGTAGGCACCATCAATAGATCGG702140.4369018881038599No Hit
TAGGGAAACTGGTTAATATTCCAGTACCTTCTTGCCTGTAGGCACCATCA641720.3993059498590152No Hit
AACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG588180.36599104529713206No Hit
TAGTCCTCGCTTTTCTCGACCGCTGTAGGCACCATCAATAGATCGGAAGA509370.31695205335611576No Hit
ACAATGGGACGAAGGACCCGTCAACGGCGGGGGCTGTAGGCACCATCAAT458620.2853732075115963No Hit
ACGAGTGTCGCTGACGCGAAAGCGTGGCTGTAGGCACCATCAATAGATCG457620.284750964243724No Hit
ACAATGGGACGAAGGACCCGTCAACGGCGGGGGTCTGTAGGCACCATCAA436730.27175230237787157No Hit
TAGGCGGCCAGAGCGGTGAGGCTGTAGGCACCATCAATAGATCGGAAGAG378040.23523284498644598No Hit
ACCTGAGCGCCTCCGGCGTTCCGGCGGCCTGTAGGCACCATCAATAGATC365420.2273801349458975No Hit
TCGAAGCATGACCTCTGATGAGATAGTTCGTGAGGCTGTAGGCACCATCA345930.2152526136550663No Hit
ACGCTTTAGGAAACACCGAGCGGGGCCCTGTAGGCACCATCAATAGATCG325920.20280152586494146No Hit
ATGGGACGAAGGACCCGTCAACGGCGGGGGCTGTAGGCACCATCAATAGA322580.20072323335024797No Hit
ACGGAAGTCTGACAGTGACATTCCTGTAGGCACCATCAATAGATCGGAAG321510.2000574330536246No Hit
TTAACGGACCTGGGTCTTATGTGAAGTCTGTAGGCACCATCAATAGATCG294150.18303285724463836No Hit
ATCGACCCGGTGGCTTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCA260040.16180813937751404No Hit
AAGCATGACCTCTGATGAGATAGTTCGTGAGGCTGTAGGCACCATCAATA258640.16093699880249285No Hit
ACAGCCGGGAGGTGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC250010.15556703940075484No Hit
AGCAAGAAGCTCGTACCGAGCTGTAGGCACCATCAATAGATCGGAAGAGC244930.15240604359996354No Hit
AACTTGGCAACAGCTGGTTCCAACCGAAACATGCTGTAGGCACCATCAAT243560.15155357032297848No Hit
ACGGGGCGCAGCAGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCA224380.13961894444518766No Hit
TAAGCCAAGGCCTGTCGGGATCAACCGACGTTAGGGCTGTAGGCACCATC222320.13833712331337075No Hit
ATGGGACCCGTAGATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC212190.1320337990098243No Hit
ACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA211550.13163556331838602Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT204310.12713052205899053No Hit
TATCCTCTGGGAAATCCAGTTCGCCGCCCCTCTGTAGGCACCATCAATAG202420.1259544822827119No Hit
ACGGACCTGGGTCTTATGTGCTGTAGGCACCATCAATAGATCGGAAGAGC200780.12493400332340131No Hit
TAAGCATGACCTCTGATGAGATAGTTCGTGAGGCTGTAGGCACCATCAAT199630.12421842356534815No Hit
ATATCGACCCGGTGGCTTGCTCTGTAGGCACCATCAATAGATCGGAAGAG191040.11887335389432505No Hit
ACGCCGGACATCTCACCAGCTCCTGTAGGCACCATCAATAGATCGGAAGA186990.1163532686594422No Hit
ATGTACACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA186190.11585547404514436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTATGACCCTCTTAAGGTAGCGTAGCTGTAGGCACCATCAATAGATCGG184020.11450520615386146No Hit
TAGCCGAGGGTTAGTCGATCCTGTAGGCACCATCAATAGATCGGAAGAGC182370.11347850476187216No Hit
TCAAGCCAAGGCCTGTCGGGATCAACCGACGTTAGGGCTGTAGGCACCAT181760.11309893636847006No Hit
TCGACCCGGTGGCTTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC180960.11260114175417223No Hit
TCGACCCGGTGGCTTGCTACCTCGCTGTAGGCACCATCAATAGATCGGAA180280.11217801633201904No Hit
ACGAGTGTCGCTGACGCGAAAGCGTGGTCTAGCGAACCCTGTAGGCACCA177760.11060996329698085No Hit
ACGATGCTCGCTAGGTGTGACACAGGCCTGTAGGCACCATCAATAGATCG170250.10593691635525984No Hit
AAACAGCCGGGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT166430.10355994707198764No Hit
TTGAAGATTGTCTCGAGCCCTCTGTAGGCACCATCAATAGATCGGAAGAG164130.10212878755588134No Hit
TAACAGCCGGGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT160830.10007538477190273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)