FastQCFastQC Report
Tue 18 Jul 2023
SRR10294598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10294598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15432004
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA15342659.94209825243695Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGCCGGGCAGCTACGCACCACGGGGTAAGAGCTGCTGTAGGCACCATCA2311791.4980491192200314No Hit
ATGTACACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA901260.5840200663504235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCCGAGGGTTAGTCGATCCTAAGTCTAACCGCTGTAGGCACCATCA713080.462078677532743No Hit
ACAATGGGACGAAGGACCCGTCAACGGCGGGGGTCTGTAGGCACCATCAA637480.41308957670047264No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC584360.37866760532203075Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCGGACATCTCACCAGCTCCGACTACAGTGCTGTAGGCACCATCAAT483230.3131349628991802No Hit
TAGGCGGCCAGAGCGGTGAGGCTGTAGGCACCATCAATAGATCGGAAGAG418680.2713063060377641No Hit
ACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT409820.26556499078149537No Hit
ACGCTTTAGGAAACACCGAGCGGGGCCCTGTAGGCACCATCAATAGATCG408380.26463186505135694No Hit
ACGAGAGCGTCACTGTCCCAACGTTGGGCCCGGTGCTGTAGGCACCATCA402550.2608540018522546No Hit
ACGCTTTAGGAAACACCGAGCGGGGCCTGTAGGCACCATCAATAGATCGG389450.2523651497239114No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA380000.24624151211987763Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAATGGGACGAAGGACCCGTCAACGGCGGGGGCTGTAGGCACCATCAAT365570.2368908146991149No Hit
TTGCCGGGCAGCTACGCACCACGGGGTAAGAGCTGCTGTAGGCACCATCA356750.23117541960201668No Hit
TCGCCCCGTCGAATCACCGAAATTCGTGGAAGCCGTCTGTAGGCACCATC342200.22174696170374242No Hit
TCAGCCGAGGGTTAGTCGATCCTAAGTCTAACCGCTGTAGGCACCATCAA319940.2073223931253517No Hit
TAGGGAAACTGGTTAATATTCCAGTACCTTCTTGCCTGTAGGCACCATCA302890.19627392527892035No Hit
ACGAGTGTCGCTGACGCGAAAGCGTGGTCTAGCGCTGTAGGCACCATCAA296670.1922433405279055No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT281670.18252328083896296Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAGTGTCGCTGACGCGAAAGCGTGGCTGTAGGCACCATCAATAGATCG281150.1821863187697463No Hit
ACGCCGGACATCTCACCAGCTCCGCTGTAGGCACCATCAATAGATCGGAA272680.17669772506539008No Hit
ACGGGGCGCAGCAGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCA238170.15433510774102963No Hit
ACAGCCGGGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT226280.14663034042759449Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAA222330.1440707247095063No Hit
TATAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGA216420.14024102119206294No Hit
ACAGAGTCGTCACCGGCAAGAGCCTGTAGGCACCATCAATAGATCGGAAG205610.13323609817623167No Hit
TTCGCCGCCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT191000.12376876003920163Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTATGACCCTCTTAAGGTAGCGTAGTACCTTGCCGCCTGTAGGCACCAT179710.11645279511332424No Hit
ACGTCGACGCTTTAGGAAACACCGAGCGGGGCCCTGTAGGCACCATCAAT178690.11579183105447613No Hit
TAACAGCCGGGAGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC177680.11513734703542068No Hit
ACGGAAGTCTGACAGTGACATTCCTGTAGGCACCATCAATAGATCGGAAG176350.1142755017430011No Hit
TCGAAGCATGACCTCTGATGAGATAGTTCGTGAGGCTGTAGGCACCATCA173320.1123120496858347No Hit
TAGTCCTCGCTTTTCTCGACCGTCTGTAGGCACCATCAATAGATCGGAAG170320.1103680377480462No Hit
TAAGCTTGTGCACCGTGAGGGAGCTGTAGGCACCATCAATAGATCGGAAG159890.10360935624433483No Hit
ACTATGACCCTCTTAAGGTAGCGTAGTACCTTGCCGCTCTGTAGGCACCA159530.10337607481180021No Hit
AAGCAGGCAAGGGTGAGGTTGTTCGCCCTGTAGGCACCATCAATAGATCG154820.10032397606947224No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)