FastQCFastQC Report
Tue 18 Jul 2023
SRR10294600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10294600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8365069
Total Bases418.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA145587217.40418399417865Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTCGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1506171.80054701282201Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA898661.0743007618944924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGTACACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA762840.9119350958133161Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCCGCCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG755000.9025627881850108Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA686600.8207941859176535No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC595360.7117215649984477Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA558260.6673704664002174Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG543930.6502397051357257Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTGGCTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT540760.6464501368727502Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA539710.6451949171011022Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC532910.6370658747704293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCCCGGCCTAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG522830.6250157649626082No Hit
ACCCTCGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC449360.5371862443692933No Hit
ACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT329910.39439005225181045No Hit
ACGCGGGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA291530.34850878097957116Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCTCGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT290740.34756437753233116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAGGCACCATCAATATATCGGAAGAGCACACGTCTGAACTCCAGTCA286450.3424359081795978Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACGGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC285110.3408340086614946Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGTCGGCACCATCAATATATCGGAAGAGCACACGTCTGAACTCCAGTCA279850.3345459553292388Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGTCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA276790.33088788628043597Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGTCCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA269500.32217307472299395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCCGCCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT266260.3182998251419086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC229750.27465404051060427No Hit
TCCGCCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA229650.2745344957704473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGTGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA219320.26218552411223384Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT205170.2452699433800247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA192420.23002798901001295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA184520.2205839545376135TruSeq Adapter, Index 9 (100% over 35bp)
ACGGGGTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA183850.21978300477856189Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA175810.21017160766994272Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCCCGGCGAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG167060.19971144290620912No Hit
TACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG166870.19948430789991095No Hit
ACGAGGTTGTCCCAGGTTCGAGTCCTGGCGGTGGCGCTGTAGGCACCATC165130.1974042294211799No Hit
ACGCGGGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA159230.19035108975191956Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGGCACCATCAATAGATCGTAAGAGCACACGTCTGAACTCCAGTCA155940.18641806780075573Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACCCCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC152320.18209054820707396Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGCGCGGGCCCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC150180.17953229076771512No Hit
ACGCGATGTACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAG150020.179341019183464No Hit
TACCCATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG147900.17680667069213654Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA147710.17657953568583834Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA147200.17596985751103786Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA144860.17317251059136513Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCTCGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG138690.16579660012368097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGGCACCATCAATAGATCGAAGAGCACACGTCTGAACTCCAGTCAC131990.157787102533165Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
AGGCGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC131150.15678292671584657Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGGGCGCAGCAGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCA128290.15336394714735765No Hit
CTGTAGGCACCATCAATAGCTCGGAAGAGCACACGTCTGAACTCCAGTCA122880.1468965767048664Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACGCCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA119530.1428918279096084Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCCGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA118700.14189960656630565Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGGCACCATCAATAGATCGGACGAGCACACGTCTGAACTCCAGTCA118600.14178006182614872Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AGCGGGATGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG118540.14170833498205454Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTATGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA117750.14076393153481462Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA116510.13928157675686834No Hit
TGGGGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC114680.13709390801199609Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTGGACGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC113450.13562350770806553No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA111060.1327663884183143Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCA110930.13261098025611026No Hit
TCAGCAAGCAGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT108920.13020813097895545No Hit
TCCGCCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA106340.127123876682906Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGGCCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT104220.12458952819157858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGCCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG100190.11977187516325329Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCTCGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA99560.11901874330026446Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCACCATCAAT98300.11751247957428683No Hit
TCGCCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA97980.11712993640578458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAA97340.11636485006878007TruSeq Adapter, Index 7 (97% over 35bp)
TTGTCGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA96640.11552803688768137Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA96370.11520526608925762Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGCCCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG90200.10782935562157349Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCTGCCCCAACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG89620.10713599612866313No Hit
CTGTCGGCACCATCAATAGATCGTAAGAGCACACGTCTGAACTCCAGTCA86520.10343010918379753Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACGGCGGTTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG86330.10320297417749931Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCCCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA85350.10203143572396116Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)