FastQCFastQC Report
Tue 25 Jul 2023
SRR10311823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10311823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47648459
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG36347477.628257190856896No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG15084203.165726723712093No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG3718820.7804701511962853No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2502970.5252992546936303No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1624650.34096590615868605No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1422680.29857838634403683No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1353490.28405745503752805No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1164720.244440224184375No Hit
ATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1106560.23223416312372241Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA950770.1995384572667922No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC878990.18447396168677774No Hit
AAGAGGTGCACAATCGACCGATCTGTAGGCACCATCAATAGATCGGAAGA867090.18197650421391381No Hit
AAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC828440.17386501418650285Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA786560.16507564284502885No Hit
AAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC765620.16068095717429182Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG756660.15880051860648842No Hit
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG697780.14644335087520877No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA677010.14208434316836982No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA669030.1404095775689199No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC595390.12495472308978554No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC593460.12454967326435468No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC567080.11901329274887987No Hit
AATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT535800.11244854739163757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA511910.10743474411208136No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC479620.10065802967520943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)