FastQCFastQC Report
Tue 25 Jul 2023
SRR10311824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10311824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47641861
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG17250313.6208304289372744No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG5339091.1206720073340544No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC3465420.7273897214048797No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2340640.49129902797038094No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2259280.47422160943712927No Hit
ATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2233940.46890275759798716Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG2213480.4646082150317344No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2022200.42445864992553506No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1863230.3910909357634035No Hit
AATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGA1617300.3394703661974917No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1487980.31232617046592703No Hit
AATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1306740.27428399574903256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1255750.26358122324398703No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG1197420.25133778884078434No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1166440.24483510415346704No Hit
AAATATAGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAGC1031290.21646719467990552No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1004470.21083769166783806No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG885000.18576100543175675No Hit
AATATAGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA836040.17548432879227785No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA795680.1670127873468251No Hit
ATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGA708150.1486402892615803No Hit
ATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG688420.1444989732873785Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC681780.14310524099803743Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA675630.1418143594348676No Hit
TAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA673200.14130430379283462No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG651490.13674738692512453No Hit
ATATAGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC643220.13501151854668314No Hit
ATGTAAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGA631650.13258298201239452No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC591300.1241135395613534No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC567020.11901718112984713No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA556590.11682792995848755No Hit
TATAGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA533140.11190578806314891No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATAG490090.10286961712096007No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCACTGTAGGCACCATCAATAG486890.10219793890922943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)