FastQCFastQC Report
Thu 24 Aug 2023
SRR10311826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10311826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30384831
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1101603436.25504449901334No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG395513213.016797756749082No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG6413892.1108855270578926No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2284450.751838968595876No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1891080.6223763429850901No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA1749700.5758465465876706No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1712300.5635377731737261No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1544750.5083951265024313No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1426920.46961590801673375No Hit
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1423440.46847059968837745No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1397070.45979192709678063No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA1285940.42321775625475755No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC987280.324925289201049No Hit
ATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT877100.2886637743682037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA855740.28163395083553366No Hit
AAGAGGTGCACAATCGACCGATCTGTAGGCACCATCAATAGATCGGAAGA827630.2723826240797588No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG707420.2328201200131737No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC693730.22831458236512817No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG631880.20795903060971443No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG571920.18822549975677008No Hit
NGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG517920.17045347397193028No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA429500.14135342730719813No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT415810.13684788965915262No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA403510.13279981711927244No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG390690.12858060655331602No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA362360.11925687524804729No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC341900.11252325214512464No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC329470.1084323950987254No Hit
AAGAGGTGCACAATCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCA313030.10302180058200752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)