FastQCFastQC Report
Tue 25 Jul 2023
SRR10311827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10311827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31339733
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG16903955.393776009514823No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG5940271.8954437167668277No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG2496620.7966309093954311No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA2189070.698496697467078No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA1980190.63184648063211No Hit
AGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGC1808300.5769991722648052No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1684500.5374966021567573No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1284560.40988224117927236No Hit
AATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGA1161880.370737044888034No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATAG1103090.3519781103431864No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG964450.30774033716241295No Hit
ATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC951760.3036911641844556No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATA937730.2992144189613868No Hit
AATTTGGAGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT934370.2981422975109584No Hit
ATGTAAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGA921260.29395910935169745No Hit
ATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT877740.28007258389852907Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG875580.27938336296611077No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG873330.2786654244948417No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAA845140.26967045315925314No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGA753650.2404774794986288No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC752760.24019349494777126No Hit
AATGAAGCGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC720290.22983284509794644No Hit
ATTAATCAAGAACGAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC669800.21372230580266907No Hit
AAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGC605400.19317331133612403No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAAT590860.18853383339290095No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG574300.1832498062443608No Hit
AATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT543370.17338054539264902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAG514170.16406329945440187No Hit
AAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC507450.161919056553545No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATAGA491170.1567243728592072No Hit
TTTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGG489410.1561627854327923No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG484380.15455779409479972No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATAGA435200.13886525453168347No Hit
TAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAG400520.12779942956118995No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGGAA394160.1257700568157361No Hit
AGGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATC366930.11708140589455564No Hit
AATTTGGAGAGGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG359520.11471699519584291No Hit
TGGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATC351190.11205902743332243No Hit
AGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT345050.11009985311617046Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT329470.10512852805733858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG323000.10306405609773384No Hit
TAGTGATGGAAAGATGAAAAGAACTTTCTGTAGGCACCATCAATAGATCG320790.10235888097706511No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACAC318280.10155798072689387No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA317250.10122932444893515No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG314460.10033908074456155No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)