FastQCFastQC Report
Tue 18 Jul 2023
SRR10311828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10311828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58019353
Total Bases8.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA8484971.4624378868892247No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG6998061.2061596067781037No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA6552871.1294283133422738No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG5145470.8868540812580243No Hit
ATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3397630.5856028763367975No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG3031410.5224825585352529No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG2861460.49319060831305717No Hit
ATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2705000.46622374434268515Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2525950.4353633519491333No Hit
AATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGA2429100.41867064598255693No Hit
AGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGC2421480.41735729110939934No Hit
ATGTAAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGA2381320.41043546280152415No Hit
AATTTGGAGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2250540.3878947081674627No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATA2075970.35780647191980924No Hit
TAGTGATGGAAAGATGAAAAGAACTTTCTGTAGGCACCATCAATAGATCG1944260.3351054259429608No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGA1854600.3196519616480384No Hit
TTTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGG1779680.3067390289581478No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1759320.3032298550450916No Hit
ATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1705040.2938743560273759Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGAAGCGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1633160.28148538643648785No Hit
TAGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1580870.2724728764210797No Hit
TGGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATC1576930.27179379266776726No Hit
TCTACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCG1507410.25981158390373643No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1473850.2540273070608009No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1459510.2515557179687957No Hit
AACGAGGAATTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1417180.244259876527751No Hit
AATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1399910.2412832835278256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAA1327710.22883915992651627No Hit
AAATGAAGCGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1300780.2241976052369974No Hit
AGGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATC1273070.21942161264707657No Hit
TAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAG1214510.2093284287399758No Hit
AAATTTGGAGAGGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1195150.2059916111094862No Hit
AAATATAGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAGC1192150.20547454226178632No Hit
AAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1126750.19420244138192994No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAAT1114840.19214967805656158No Hit
AATTTGGAGAGGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1112380.1917256816014477No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATAG1051040.18115334722881174No Hit
TATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGAAG1015680.17505882907725634No Hit
ACCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG995170.17152380172181514No Hit
AGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT970340.16724419522568618No Hit
TATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT961730.16576020763278762No Hit
AAATTTGGAGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG932270.16068259154837525No Hit
AGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT931420.16053608870819364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCATTGTAATTATTGCTCTTCCTGTAGGCACCATCAATAGATCGGAA878700.15144946549128185No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATC863260.14878828448845338No Hit
AAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAG857990.1478799668793273No Hit
ATTAATCAAGAACGAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC846340.1458720161874263No Hit
AAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGC842870.14527393988692017No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG833920.14373135115794897No Hit
AACGATCAACTTAGAACTGGTCTGTAGGCACCATCAATAGATCGGAAGAG811350.13984126986042053No Hit
TTTGGAAGAGATGTATTTATTCTGTAGGCACCATCAATAGATCGGAAGAG806890.13907256084017347No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG800130.13790743237002318No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGGAA788190.1358494983561778No Hit
ACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGAA770730.13284015766256477No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATAGA749450.12917241596954726No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC739720.12749538934017413No Hit
GGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGA736510.12694212567313531No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA732450.12624235916591486No Hit
TTGGGTAAAACCATACTGCAGACATCTCTGTAGGCACCATCAATAGATCG717460.12365873849024135No Hit
AAAATTTGAAATCTGGTACCTCTGTAGGCACCATCAATAGATCGGAAGAG707000.12185589177459459No Hit
TAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGC697940.12029434385454109No Hit
TTAACGATCAACTTAGAACTGGTCTGTAGGCACCATCAATAGATCGGAAG672160.11585099888997384No Hit
AGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC649240.11190059289354708No Hit
AGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGA643370.11088886151488107No Hit
AAAAAGGTGTTAGTTCATCTAGACCTGTAGGCACCATCAATAGATCGGAA638650.11007533986116667No Hit
AGTGATGGAAAGATGAAAAGAACTTTCTGTAGGCACCATCAATAGATCGG635860.10959446583280583No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG613100.10567163684158973No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACAC602420.10383087174377832No Hit
TATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC600520.10350339480690177No Hit
ATTCAATGAAGCGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA590770.1018229210518773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)