FastQCFastQC Report
Tue 18 Jul 2023
SRR10311829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10311829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45961725
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG22423134.878652835593094No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG4495750.978150841814575No Hit
ATGTAAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGA2473510.5381673555550841No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA2032100.4421287495193011No Hit
TGGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATC2000910.4353426682745263No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG1823780.39680407991649574No Hit
AATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGA1802540.39218284344201615No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA1776320.38647809672069533No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG1690480.36780168716470063No Hit
TAGTGATGGAAAGATGAAAAGAACTTTCTGTAGGCACCATCAATAGATCG1467010.3191807966302396No Hit
AGAGGTGAAATTCTTGGATTTCTGTAGGCACCATCAATAGATCGGAAGAG1212280.26375859478729313No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1093800.237980624095375No Hit
AGGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATC1042010.22671255267290336No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG991570.21573820390770798No Hit
AAAATTTGAAATCTGGTACCTCTGTAGGCACCATCAATAGATCGGAAGAG873640.1900798980020876No Hit
AAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC868440.18894852184072725No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATA859010.18689681468656802No Hit
TGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATCG842170.18323289650247027No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG806180.1754024680318243No Hit
AAATCGATCGTCGAATTTGGGTCTGTAGGCACCATCAATAGATCGGAAGA748320.1628137325133032No Hit
ATTAATCAAGAACGAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC710020.15448071194020677No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATAGA709240.1543110055160027No Hit
AGTGATGGAAAGATGAAAAGAACTTTCTGTAGGCACCATCAATAGATCGG690810.1503011473133352No Hit
ATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT679210.1477773081841467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATAG673420.1465175643429397No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATAGA654090.1423118910354213No Hit
AAATATAGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAGC632180.13754488109399723No Hit
AAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAATAGATCGGA624510.1358761012559907No Hit
AATTTGGAGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT595380.12953821902898555No Hit
ATTTAGAGGAACTAAAAGTCGTCTGTAGGCACCATCAATAGATCGGAAGA571290.12429690138914498No Hit
AGAAAGTGATGTTGACGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC569490.12390527117944333No Hit
TTGGGTAAAACCATACTGCAGACATCTCTGTAGGCACCATCAATAGATCG549980.11966043485095477No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT545760.11874227958154311No Hit
GACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATCGG524880.11419936914900386No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCCTGTAGGCACCATCAATAGATCG519180.11295920681828195No Hit
ACCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG497490.10824006279137696No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC497360.10821177838734296No Hit
TATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGAAG488080.10619270708399217No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC487310.10602517638317534No Hit
ATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC484250.10535940502668253No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG476680.10371238242254832No Hit
AAAAAGGTGTTAGTTCATCTAGACCTGTAGGCACCATCAATAGATCGGAA465850.10135607399417669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)