FastQCFastQC Report
Tue 18 Jul 2023
SRR10311830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10311830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59203701
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1930499232.60774524889922No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG26481144.472885909615684No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC5213780.8806510255161245No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC4154120.7016655935074059No Hit
TGGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATC3061730.5171517908990183No Hit
AATTTGGAGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2838880.4795105630305105No Hit
AATTTGGAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2617610.4421362103696862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2560310.43245776138218117No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2535320.4282367414834421No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA2163150.36537411740526154No Hit
AGGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATC1821710.3077020472081636No Hit
CGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1586200.26792243951100286Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1300660.2196923465983993No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA1262030.21316741667889985No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1232560.20818968733052687Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA1144540.19332237354553222No Hit
AAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1099810.18576710263434376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1050070.17736560084309594No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG994290.1679438925617167No Hit
AAATTTGGAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG979710.16548120868322067Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA979590.16546093968010547No Hit
AAATTTGGAGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG977690.16514001379744825No Hit
TGACTTTTACTTTGAAAAAATTAGAGTCTGTAGGCACCATCAATAGATCG936410.1581674767258216No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC912490.15412718877152629No Hit
ATGGCTGGAAGAGGCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA852000.1439099221178757No Hit
TGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAATAGA826460.1395960026215253No Hit
AATGAAGCGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC809740.13677185485414162No Hit
AAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC737570.12458173856394553Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA725890.12260888892739999No Hit
AGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT722850.12209540751514843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAATA711290.12014282688171808No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATAGA710350.1199840530239824No Hit
TAGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC701580.11850272671298033No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT679760.11481714631320092No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC676480.11426312689471897No Hit
TAGTGATGGAAAGATGAAAAGAACTTTCTGTAGGCACCATCAATAGATCG653190.11032925120677844No Hit
AAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT643270.1086536802825891Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAGGCCTCAGGATCTGCTTCTGTAGGCACCATCAATAGATCGGAAGA611750.10332968879766485No Hit
AATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT601370.1015764200282006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTGGAGAGGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG592160.10002077403910947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)