FastQCFastQC Report
Wed 27 Sept 2023
SRR10315302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114118245
Total Bases11.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA73643266.45324154783488Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC55182494.835553683812786No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT53710994.706608483157098No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA23550792.0637182073734133No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG22074421.9343462563764453No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT9599790.8412143036374244No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC8985100.7873499982408598No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA4228400.3705279554553262No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG4060600.35582390878864284No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC3882140.34018574330511303No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT3659320.32066038169444333No Hit
AAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3606750.31605375634719934Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3496610.30640236361854317Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA3409520.2987708056674023No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA3270940.28662726104839764No Hit
TAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACTGTAGGCACCATCA3249080.28471170407501445No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2873140.25176868080997916No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG2831270.248099679415855No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA2764960.24228903975871693No Hit
TTGATGATTCATAATAACTTTCTGTAGGCACCATCAATAGATCGGAAGAG2515400.22042049454931595No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2471650.21658675175034456Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCGG2462060.21574639532881004No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGAT2445560.21430052661605514No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAG2371060.20777221030694962No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATA2295410.2011431213299854No Hit
TTTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA2184460.19142074959179403No Hit
TAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTGTAGGCACCATC2174640.19056023863668778No Hit
AAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCG2086790.18286208309635327No Hit
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2067000.1811279169251157No Hit
TCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2002290.17545748271891143Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGA1909990.16736938076816726No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG1908050.16719938165891002No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1861690.16313692871810287No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA1839210.16116704213248284No Hit
TGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTCTGTAGGCACCAT1827880.1601742122830578No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGAT1824710.1598964302333952No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1781060.1560714502751072No Hit
ATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1767910.15491913672524496No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA1737690.15227100627073262No Hit
ATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGAA1697400.14874045775940561No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1645010.14414960552539166No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTTC1608670.1409651892210575No Hit
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1547490.13560408329097595Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATCTGGTTGATCCTGCCAGTAGTCTGTAGGCACCATCAATAGATCGGAA1510140.13233116229573982No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTT1488030.13039369822064825No Hit
CCTGCGGAAGGATCATTAAAGACTGTAGGCACCATCAATAGATCGGAAGA1427440.12508429305059854No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTG1412170.12374620727824898No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATA1399320.1226201822504368No Hit
TTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1323560.11598145414872091No Hit
GAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1280370.1121967832575764Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGT1274460.11167889937318963No Hit
AGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGA1244210.10902814006647228No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG1218410.10676732717016459No Hit
ATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGA1163700.10197317703229664No Hit
CTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATCG1163400.10194688851024654No Hit
AGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTAATTCAGGGAGGTAGT1160470.101690137278224No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA1143520.10020483578239395No Hit
GTCGGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1142510.10011633109149198Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)