FastQCFastQC Report
Wed 27 Sept 2023
SRR10315303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124346987
Total Bases12.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC28454022.2882757907113582No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT23061851.8546368156069595No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22052971.7735025618272522Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT10005430.8046379121353378No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA8858500.7124016603635116No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA8395190.6751422131362137No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG7966010.6406275047098648No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7051160.5670551551039994No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA6578230.5290220662926075No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA5568390.44781060919473664No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA5118310.4116151201958757No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC4992260.4014781636807975No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA4737220.3809678154887661No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT4457530.35847511126264764No Hit
ATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGAA4392250.353225285627548No Hit
TATCTGGTTGATCCTGCCAGTAGTCTGTAGGCACCATCAATAGATCGGAA4017490.3230870402995772No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACC3593020.28895111065296664No Hit
TTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3532030.2840462873459089No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT3337730.2684206574301635No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA3228320.25962189176324796No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC3138490.2523977521063699No Hit
CCTGCGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2951000.23731978322884495Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT2878280.2314716318779803No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA2813930.22629659695735127No Hit
AAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2708230.21779618994708733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2626770.2112451667204449No Hit
AAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCG2580040.20748713436860355No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2459080.1977595162800366No Hit
ATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGAA2450980.19710811328303435No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC2359120.1897207207762903No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA2258280.1816111555642277No Hit
TATCTGGTTGATCCTGCCAGTACTGTAGGCACCATCAATAGATCGGAAGA2181160.17540915567178156No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA2155800.1733697013503029No Hit
CCTGCGGAAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2143490.17237972963510567No Hit
ATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGA2000350.16086839321647575No Hit
AGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGA1937210.15579066664478167No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG1931120.15530090809518368No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1927810.1550347174877667No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAG1906810.15334589490294606No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC1891150.15208651577540838No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGG1729600.13909464489075238No Hit
TTGATGATTCATAATAACTTTCTGTAGGCACCATCAATAGATCGGAAGAG1709730.13749669704501968No Hit
ACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGAAGA1684250.13544759230877063No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGAT1650780.13275593078905884No Hit
AGAGTGTTCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGC1605140.12908555637138197No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGAT1560330.12548193065586702No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGA1534200.12338055283961163No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA1506330.12113924400918535No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAG1469470.11817495827220968No Hit
CTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCGG1447820.11643386260738267No Hit
TTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1418410.11406870678740288No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG1411390.11350415752333427No Hit
ATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1387640.11159417960002521No Hit
CCTGCGGAAGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1369130.11010560312169043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGATGATTCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1367010.10993511246074664No Hit
AATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGG1309810.10533508142018752No Hit
TACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGAAG1298860.10445448107238818No Hit
TATCTGGTTGATCCTGCCAGTAGTCATCTGTAGGCACCATCAATAGATCG1262200.10150627936002984No Hit
TCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1255260.10094816370580817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)