FastQCFastQC Report
Wed 27 Sept 2023
SRR10315304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140328919
Total Bases14 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1660234711.831023226224668Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC44263043.1542350867820765No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT21815271.5545812050330126No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC12822910.9137752995873929No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG10435000.7436100893786548No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT7716120.5498595766992261No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA5803920.4135940076613859No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4794080.3416316489974529Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4381840.3122549529509309No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4093700.2917217654901197Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA3724910.2654413663658308No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA3216600.22921861173889607No Hit
TATCTGGTTGATCCTGCCAGTACTGTAGGCACCATCAATAGATCGGAAGA3020800.21526567877288358No Hit
CCTGCGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3012720.21468988868930147Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA2922100.20823220337071074No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2919370.20803766043405494No Hit
TATCTGGTTGATCCTGCCAGTAGTCTGTAGGCACCATCAATAGATCGGAA2867370.20433208068822936No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2623590.18696003779520312No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA2595050.18492624460393656No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA2541190.18108811912104875No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC2482510.17690651490018247No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC2196630.15653437763601669No Hit
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2173160.15486187846996813No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2120670.151121380761153Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT2109390.1503175550009047No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA2085470.1486129883178249No Hit
NTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2064050.1470865745071406Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA1993510.14205981305963028No Hit
AATGACCCACTCGGCACCTTACGAGAAATCAAAGTCTTTGGGTTCTGGGG1922190.13697746791593257No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1704820.12148743196689202No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1649270.11752887514226487No Hit
CCTGCGGAAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1614290.11503616015170756No Hit
GCGGCGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1587920.11315700365368025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG1564590.11149448104848582No Hit
TAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACTGTAGGCACCATCA1522600.10850222540373165No Hit
CCTGCGGAAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1493140.10640287195542353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG1472390.10492420311454119No Hit
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1470100.10476101508342696Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)