FastQCFastQC Report
Wed 27 Sept 2023
SRR10315305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99998928
Total Bases9.9 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA42736324.273677813826164Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC29045362.904567136959708No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT22400452.2400690135398253No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA10453641.0453752064222128No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG9822640.9822745299829614No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT9412610.9412710904260893No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC8403880.8403970090559371No Hit
GCGGCGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT6545110.6545180164331361Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA5930870.5930933579607974No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5432300.5432358234880278No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC5287820.5287876686038074No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT5123370.5123424923115176No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA4286230.4286275948878172No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA4223230.4223275273510932No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA3272770.32728050844705053No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA3201000.3201034315087858No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGG2862360.28623906848281416No Hit
TATCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG2564620.2564647493021125No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA2284540.22845644905313384No Hit
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2259980.22600042272453164No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2055280.20553020328377922No Hit
TTGATGATTCATAATAACTTTCTGTAGGCACCATCAATAGATCGGAAGAG1992980.19930013649746325No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1829970.18299896174886995No Hit
TGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGAT1817930.18179494884185157No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTTC1765800.1765818929578925No Hit
CAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1709250.17092683233564263Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1590700.15907170524868025No Hit
TATCTGGTTGATCCTGCCAGTACTGTAGGCACCATCAATAGATCGGAAGA1575720.15757368918994813No Hit
ATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGAA1556900.15569166901469184No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1506770.15067861527475573No Hit
TTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1482670.1482685894392788No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1465010.14650257050755586No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGGC1447750.1447765520046375No Hit
ACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCAC1429960.14299753293355305No Hit
CCTGCGGAAGGATCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1403310.14033250436444678No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA1392110.13921249235791808No Hit
TATCTGGTTGATCCTGCCAGTAGTCTGTAGGCACCATCAATAGATCGGAA1378630.13786447790720316No Hit
ATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1293370.12933838650750337No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTT1291030.12910438399899646No Hit
GCGGCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1276090.1276103679831448Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTCGAA1228130.12281431656947363No Hit
GCGGCGCTACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1178290.11783026314042086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC1165500.11655124942939388No Hit
CTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT1158480.1158492419038732No Hit
TTTGATGATTCATAATAACTTTCTGTAGGCACCATCAATAGATCGGAAGA1146010.1146022285358899No Hit
ATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGA1094480.10944917329513773No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTG1075170.10751815259459581No Hit
AGAGCTAATACATGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1046500.10465112186002633No Hit
CCTGCGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1010240.10102508298888964Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1009360.10093708204551952No Hit
TTTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1003530.10035407579569253No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTCGAAT1000110.10001207212941322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)