FastQCFastQC Report
Wed 27 Sept 2023
SRR10315306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94091470
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT66132767.028560612348814No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA43455074.618385704889083Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC23663952.5149941859766884No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA23438042.4909845706523663No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG22909342.43479456745654No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC22231302.3627327748200764No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT13088541.391044267881031No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT9089990.9660801345754297No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA8288190.8808651836346058No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC6387760.6788883200570679No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGAT6112050.64958598266134No Hit
AAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG5300570.5633422455829418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA5220590.5548420064007927No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA4514960.47984796071312313No Hit
GCGGCGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3848340.4089998806480545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGG3549820.37727330649632745No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA3302860.35102650644101957No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA3222320.3424667507054572No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2878740.30595121959514504No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTTC2775600.29498954581111336No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA2547020.2706961640624809No Hit
TTGATGATTCATAATAACTTTCTGTAGGCACCATCAATAGATCGGAAGAG2487550.2643757186490975No Hit
TGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGAT2128030.2261660913576969No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTCGAA2060520.21899115828459265No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGGC2008480.21346036999953344No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTT1908660.20285154435359548No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1891840.20106392216000024No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1851890.19681805375131242No Hit
TTTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1817930.1932087999050286No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTCGAAT1817740.1931886067886919No Hit
NTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT1787510.18997577569996515No Hit
ACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCAC1759310.18697869211736196No Hit
CTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT1594320.16944362756794001No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGT1576780.1675794840913847No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1395600.14832375347095758No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTG1395320.14829399519425088No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT1313720.13962158312544165No Hit
TCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1309270.13914863908492447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1308790.13909762489628444No Hit
CCTGCGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1303750.13856197591556385Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1284940.13656285739823174No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCT1267730.13473378617636647No Hit
GAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1259100.13381659357644216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1245070.13232549135431723No Hit
AGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTAATTCAGGGAGGTAGT1208650.12845478979125313No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC1207140.1282943076561563No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1111860.11816799121110552No Hit
ATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA1087000.1155258813577894No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1073030.11404115590924449No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC1063830.1130633839603101No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAGA1039910.11052117689308075No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA1020480.10845616504875522No Hit
GAAGGCAGCAGGCGCGCAAATTACCCAATCCTAATTCAGGGAGGTAGTGA1010900.10743800686714748No Hit
CGGCGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG979920.10414546610867065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
NTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA979620.10411358224077061Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)