FastQCFastQC Report
Wed 27 Sept 2023
SRR10315307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111944323
Total Bases11.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT48257324.310832269716794No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA32256402.8814681384066256Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC30366642.7126556475758044No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG29709772.653977370518378No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT22005041.965712901760994No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC20388511.8213080800890635No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA15873401.4179727541878118No Hit
GCGGCGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT9411190.8407027482760336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTTC5502830.4915684737313566No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5212890.4656680982384431No Hit
TGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGAT4930120.4404082197182969No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA4149280.3706556874706366No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTT3489960.31175855161498456No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA3157610.2820696856597185No Hit
TTGATGATTCATAATAACTTTCTGTAGGCACCATCAATAGATCGGAAGAG2916890.26056613875810386No Hit
CCTGCGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2668910.23841405517276656Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT2668360.23836492360581785No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGT2443950.21831835098953611No Hit
CAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC2298340.2053109919651754Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG2279410.2036199727609233No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA2169710.19382045840770326No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT2162380.19316566861545986No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTG2126590.18996854355892617No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1901440.169855866652568No Hit
AAGGCAGCAGGCGCGCAAATTACCCAATCCTAATTCAGGGAGGTAGTGAC1823700.1629113429896753No Hit
GCGGCGCTACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1787760.1597008184148829Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1764120.15758905433730658Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG1694970.1514118764200307No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTGTA1641540.14663896801627002No Hit
AGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTAATTCAGGGAGGTAGT1617710.14451023121556597No Hit
ATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCT1590370.14206794568760758No Hit
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1590330.1420643724827386No Hit
TGAAGGCAGCAGGCGCGCAAATTACCCAATCCTAATTCAGGGAGGTAGTG1554250.13884134169090467No Hit
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCA1551950.13863588241093744No Hit
CCTGCGGAAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1533000.13694307660425087Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1504950.13443736668986778No Hit
CAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGAT1499780.13397552996055012No Hit
TATCTGGTTGATCCTGCCAGTACTGTAGGCACCATCAATAGATCGGAAGA1461150.13052470735831775No Hit
ATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA1432480.12796361276846527No Hit
TGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTTCT1398880.12496212067850908No Hit
GAAGGCAGCAGGCGCGCAAATTACCCAATCCTAATTCAGGGAGGTAGTGA1383260.12356678417716636No Hit
AAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCA1357620.12127635985614027No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA1319020.11782821715755964No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATA1312080.11720826611278895No Hit
TTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1300340.11615953048373878No Hit
CCTGCGGAAGGATCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1292520.11546096893185016No Hit
TTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGA1225800.10950086321036573No Hit
GCGGCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1196590.10689153035478181Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATCTGGTTGATCCTGCCAGTAGTCTGTAGGCACCATCAATAGATCGGAA1185220.10587584687076987No Hit
AAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCG1163500.10393559662690534No Hit
AATGACCCACTCGGCACCTTACGAGAAATCAAAGTCTTTGGGTTCTGGGG1123760.10038561758955833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)