FastQCFastQC Report
Wed 27 Sept 2023
SRR10315308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127214658
Total Bases12.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA83003096.524648283847919No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC48056103.777559972688053No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT43202163.3960048849087814No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA34902592.743598147314125No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA28208842.2174205742863373Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGC27868272.190649288229034No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA18666171.4672971097402943No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG17905801.4075264817360904No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC15150611.1909484518678657No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG9651510.7586790824057398No Hit
TGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGC9369690.7365259748605385No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC9319200.7325570925954146No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG8615030.6772041945040641No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG8156520.6411619642132749No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTT7495120.5891711000787346No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC7486460.5884903609142274No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG7058260.5548307176992135No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACC6728880.5289390472597898No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA6256000.4917672301567639No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC5829680.4582553686541373No Hit
CACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGA5792270.45531466979221846No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGG5280700.41510153649118015No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG5156030.4053015651702652No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGAC5059890.3977442599421208No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4943020.3885574255130254Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCA4911020.386041992110689No Hit
CACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAG4521460.3554197347289964No Hit
TTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTG4415540.34709365016726296No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACACTGTAGG3978380.31272968559959496No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA3955060.31089656350764233No Hit
CAGGCCACCATCGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATACT3949110.3104288501093954No Hit
TTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAG3849350.30258698647761173No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA3640160.2861431266827758No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA3591650.2823298868594215No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT3510940.27598549217496615No Hit
TCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGA3473670.27305579833418253No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA3316100.26066964704649054No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG3155570.24805081816908237No Hit
TTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCAC3088180.24275347263834957No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA3047140.23952742929985318No Hit
TGTTGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTA2793480.2195879031487079No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA2733750.2148926894886594No Hit
GTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA2702140.212407912930914No Hit
TGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGAG2694750.2118270050295619No Hit
CCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA2628850.20664678436662542No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC2557860.2010664525781298No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT2469790.1941435082111371No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCT2421690.19036249737825023No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC2390020.18787300438287544No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGG2360200.18552893488107322No Hit
TGCAGGCCACCATCGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATA2273280.178696388901977No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT2244900.1764655139032799No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG2223840.17481004429536728No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2215580.1741607480483892No Hit
TTGCTCCACACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2207860.17355389974007554No Hit
TGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC2129160.16736750571620448No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACC2103890.16538109940129697No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTCTGTAG2093210.1645415735032672No Hit
ATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGAA2068570.1626046897834682No Hit
TTGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGA2030640.15962311512876134No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCT1968350.1547266667965259No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAA1940300.15252173220479043No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGGCTGTAGGCACCATC1924400.15127187623300453No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGCT1915360.1505612662968445No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGCA1898520.149237519468865No Hit
TTGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGG1854530.14577958461359067No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT1851670.14555476775325688No Hit
CTACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGG1801970.14164798525025316No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA1797150.14126909809402624No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1773950.13944540887733237No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACC1764730.13872064962828418No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1753860.13786618834442804No Hit
GGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGC1735670.13643632166978747No Hit
CGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTG1713930.13472739910207518No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG1703870.13393660972621568No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1644350.12925790359786998No Hit
TGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAGA1638750.12881770275246113No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTG1623030.12758199609356335No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1610060.12656245949267889No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA1609660.1265310165751497No Hit
CTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCT1597250.1255555000588061No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1588840.12489441271775459No Hit
TTTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1557210.1224080640141327No Hit
TCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG1545530.12148993082227992No Hit
ATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1540650.12110632722842363No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1527070.12003884017830711No Hit
AGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGA1436960.11295553693191551No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1414570.11119551962321826No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG1405210.11045975535303486No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1384170.10880585789099868No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT1379090.1084065328383778No Hit
TTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGA1376710.10821944747907904No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG1351480.10623618545592443No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTCTGTA1336950.10509402147667606No Hit
TATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGA1335090.10494781191016525No Hit
ACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCAC1333170.10479688590602507No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC1317840.10359183609171829No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATAGATC1313230.1032294564671942No Hit
AGGCCACCATCGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATACTG1312470.10316971492388871No Hit
GCAGGCCACCATCGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATAC1306230.10267920541043314No Hit
ATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGAA1301150.10227988035781223No Hit
TTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCC1288230.10126427412161891No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA1284930.10100487005200297No Hit
AAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCG1282740.10083272007853057No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG1280930.10069044087671092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)