FastQCFastQC Report
Wed 27 Sept 2023
SRR10315309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124213175
Total Bases12.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3043053324.498635511088096Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC36295172.9220064618749175No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT33606952.705586585319955No Hit
AAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG33118132.6662332719536392Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGAT26600542.1415232321370095No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC21965971.7684090274642768Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA10780330.8678894167225014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGC9873180.7948577113498629No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAGA9577690.7710687694763458No Hit
AACCTGGATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA9452820.7610158906251289Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC9243040.7441271829658971Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA7548080.6076714487009933No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA7212420.5806485503651283No Hit
NTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7106410.572114028966734Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA5438080.43780218966305307No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC5341700.43004294834263757No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG5204650.4190094971809552No Hit
CCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAGA4748120.38225574702522497No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT4435370.357077258511426No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA4279540.34453189043754817No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACC4254230.34249426439667124No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG3956780.318547529277792Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3944470.3175564910888076Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3632660.2924536789273763No Hit
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3627290.2920213576377868Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGATAAATCCATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3537380.28478299504058247No Hit
TGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3315880.2669507481794906No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3313940.2667945650692851No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC3234690.26041440451063264No Hit
TAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGC2891380.23277562947730787No Hit
TTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCAC2890600.2327128342061943No Hit
TCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAG2684790.21614373837557893No Hit
TTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2477260.19943617092148236No Hit
CCGTGAAAATCCACAGGAAGGAACTGTAGGCACCATCAATAGATCGGAAG2428570.1955162968823557No Hit
TTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2392370.1926019522486242No Hit
ATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2357570.18980031707586575Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGATAAATCCATAGGACTGTAGGCACCATCAATAGATCGGAAGAGCACA2311240.18607043898523648No Hit
GTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC2277300.1833380396242186No Hit
TACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2124870.17106639452698957No Hit
TTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2105970.16954481680385353No Hit
CCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAG2070020.16665059885958153No Hit
ATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2013090.16206734913587065No Hit
TGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAGAGCA1970310.15862327003556587No Hit
TTGCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1942600.15639242777587803Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC1934780.15576286492958577No Hit
TTCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1922560.15477907234880678No Hit
ACCTGGATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1887210.15193315845923752Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAAATCAGTTATCGTTTATTTGATAGTTCCCTGTAGGCACCATCAATAG1848080.14878292902504103No Hit
AGCTGGGACTGAGGACTGCGACGTCTGTAGGCACCATCAATAGATCGGAA1840060.14813726482718118No Hit
TGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1828910.14723961447728875No Hit
GTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA1793000.14434861680333025No Hit
CCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAG1763540.14197688771742611No Hit
CATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAG1754520.14125071676172837No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1682680.13546711127865463No Hit
CAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1663930.13395760956919425Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGG1608260.12947579836035913No Hit
TTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1598670.1287037385526938No Hit
GAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1540420.1240142199086369Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAG1540190.1239957033543342No Hit
CTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1526600.12290161651531731No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATAGATC1524920.12276636516214967No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1524630.12274301820237668Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTATTTGATAGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1502070.12092678574555397No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1495170.12037128911647256No Hit
TGGAATAATAGAATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1493380.12022718201994273No Hit
TATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1481060.11923533876337998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT1476170.11884166071755271No Hit
CGTGTGGCGAGGAGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1454650.1171091552888814No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA1446810.11647798230743237No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCA1442370.11612053230263214No Hit
AAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCG1415140.11392833328670651No Hit
GTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1388440.11177880285243494No Hit
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1375810.11076200250094244Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1371180.11038925621215301Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1360640.1095407149845417No Hit
TTGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGG1323290.10653378757929663No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1307320.10524809465662559No Hit
ACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1278720.10294560138246205Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTAAATCAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1277130.1028175956374998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGC1251020.1007155641903526No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)