FastQCFastQC Report
Wed 27 Sept 2023
SRR10315310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108235613
Total Bases10.8 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA90900468.398387321925178Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGAT49438494.5676731188282735No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT34161253.156193146889647No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC32648063.016387960956991No Hit
AAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG22217572.05270422407087Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGGATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA19248591.7783970974507253Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAGA14622001.3509416720354326No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG9206820.8506276025803079No Hit
CCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAGA8650710.7992480256937243No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA8208210.7583649939692216No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA7807590.7213512986709836No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT7734590.7146067533243425No Hit
AGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA7218340.6669098829790893Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGC7007660.6474449403266187No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC6824660.630537381443943Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA6608110.6105301034327768No Hit
TTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC6146700.5678999572903976No Hit
CCGTGAAAATCCACAGGAAGGAACTGTAGGCACCATCAATAGATCGGAAG4963380.45857180113166635No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC4603130.42528793180115315No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA4381140.4047780465751139No Hit
TTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4343200.4012727308155034No Hit
ACCTGGATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3848940.3556075392671357Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACC3616870.3341663524370671No Hit
AGCTGGGACTGAGGACTGCGACGTCTGTAGGCACCATCAATAGATCGGAA3422050.31616673155442837No Hit
TTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCAC3374960.3118160378506841No Hit
CCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAG3314570.30623654341940115No Hit
TGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC3269780.30209834908959216No Hit
TGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAGAGCA3043200.2811643890260038No Hit
CCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAG3018700.2789008087384325No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC2692430.2487563866802325No Hit
TGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2663840.24611492707118499No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTCCCTGTAGGCACCATCAATAG2560960.23660973768402827No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2540210.23469262376700356Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCG2482110.22932470479933442No Hit
TATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2435440.22501281532909134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATAAATCCATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2376750.21959038565245617No Hit
GTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA2321710.21450518324315307No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC2305130.21297333993017623No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGC2270260.20975166463925327No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATAGATC2182520.20164527547878347No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC2114450.195356217920621No Hit
TTATTTGATAGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2063400.1906396557295795No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA2060560.19037726519828552No Hit
CGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCAC2005050.1852486390038739No Hit
TAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGC1998480.1846416299226762No Hit
TTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1988820.18374913255214806No Hit
TTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1949830.18014680620878454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1942440.1794640364812273Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1908870.17636246953209386Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1866080.1724090572665764No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1826140.1687189594426744No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1757800.16240495630583254No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA1721640.15906409658344153No Hit
TTTGAGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1681100.15531856414025205Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAA1672040.1544815013890114No Hit
TTCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1666480.15396780725028092No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1644380.15192596543985942No Hit
TTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1633740.15094292485782845No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1627710.15038580693398945Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1626080.15023520955159186No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1621820.14984162375465088No Hit
CCCGTGAAAATCCACAGGAAGGAACTGTAGGCACCATCAATAGATCGGAA1612930.149020267478875No Hit
TTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1608890.14864700770900607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1606340.14841141057703439No Hit
ATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1575410.14555375595276576No Hit
AGGATAAATCCATAGGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1557960.1439415324418221No Hit
TCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1529330.14129637719148871Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAATCAGTTATCGTTTATTTGATCTGTAGGCACCATCAATAGATCGGA1511410.13964072989543655No Hit
TACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1503050.13886834086669791No Hit
TTGCTCCACACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1498500.1384479616704347No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT1490800.13773655072291224No Hit
CGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAGAG1484540.13715818286167974No Hit
CAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAA1440470.1330865100750157No Hit
TGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCA1429880.132108089044592No Hit
ATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1424340.13159624272650444No Hit
GTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1402180.1295488574541542No Hit
GTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1390990.12851500180444306No Hit
TCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAG1387750.12821565486029077No Hit
CAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1365060.12611930234090327Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1362350.1258689226437882Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1357960.1254633260126683Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTAAATCAGTTATCGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1354970.12518707682655245No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA1345170.12428164471152392No Hit
CTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1343460.12412365604655465No Hit
TCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1273780.11768584892663748No Hit
CATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAG1261470.11654851532092306No Hit
CTACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGG1249230.11541764908745886No Hit
AAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGATCGGAAG1247560.11526335606377543No Hit
TGTTGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTA1240920.11464987961032752No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGG1180430.10906114607582995No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1158300.1070165325344441No Hit
TGAAAATCCACAGGAAGGAACTGTAGGCACCATCAATAGATCGGAAGAGC1154470.10666267488132579No Hit
GTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1132310.10461528960897556No Hit
NTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1131650.10455431152775936Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATC1126150.10404616085095762No Hit
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1110060.1025595891437322No Hit
AAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1101340.1017539393434211No Hit
CCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGGAAGA1095010.10116910410993837No Hit
TGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAGA1094730.10114323462093756No Hit
ATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1090180.10072285542467431No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG1084010.10015280275633492No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1083850.10013802019119161No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)