FastQCFastQC Report
Wed 27 Sept 2023
SRR10315311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39392771
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT32070138.14112061322114No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG15198763.8582612022901355No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA13134873.3343351245841526No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT12340593.1327042212897385No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA9223672.3414625998257397No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA6619891.680483457231277No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATAGATC6430031.6322867969861778No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA6168501.565896443283972No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA5296451.3445233390664495No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA4960451.2592285015948739No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT4521461.1477892733161625No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGA4342441.1023443869942533No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG4240111.0763675396178654No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAA3917600.9944971883293003No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAA3791420.9624659306145281No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA3612310.9169981974611534No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG3610160.9164524120428086No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA3491020.8862082842560124No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC3301040.8379811615689589No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG3256760.8267405204878835No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCG3008900.7638203466316192No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC2722440.691101420613442No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2569630.6523100393216816No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG2516170.6387390214311148No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG2448940.6216724383263112No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2374380.6027451077254758No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA2361260.5994145474051572No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG2232570.5667461169461777No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA2154260.5468668350342757No Hit
TTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGA2129510.5405839563812356No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG2068150.525007494395355No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGA1807160.45875422168194263No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1794050.45542619989845345No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGGAA1371780.34823140519868484No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGGCTGTAGGCACCATC1340910.3403949420059838No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1323570.3359931191436114No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAAT1299130.3297889351322861No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT1186500.30119739482150165No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT1177280.2988568638646923No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1102710.2799269947270274No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1018640.2585855156013269No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA899780.22841246684575703No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATAGATCGG813330.20646681595463287No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA689680.17507780805772713No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT650840.16521813101190572No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGCTGTAGGCACCATCA621750.1578335273748577No Hit
CACGTTCTAGCATTCAAGGTCCCATCTGTAGGCACCATCAATAGATCGGA606370.1539292577310695No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA559700.14208190634774082No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA535200.1358624911154384No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG523760.13295840498247763No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG522650.1326766273944019No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG517460.1313591267798856No Hit
TTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAA514180.1305264866998059No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA510380.12956184270459165No Hit
CAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGAT501100.1272060805268053No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGCTGTAGGCACCATCAA483590.12276110253833122No Hit
TCTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA481850.12231939712999626No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT475110.12060842330690572No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG466880.11851920749621801No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG455820.11571158576277865No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT435460.1105431247778939No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG432870.10988564373905049No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG409520.10395816024214188No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA394080.10003865937737663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)