FastQCFastQC Report
Wed 27 Sept 2023
SRR10315312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289863791
Total Bases28.9 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT44346201.5298978822780938No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC27286540.9413573149603912No Hit
CCTGCGGAAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC21133710.7290910647063192No Hit
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACA20858860.71960902491612No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA14372670.49584220058724066No Hit
TTTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA11136610.3842014886226338No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCCTGTAGGCACCATCAAT10872360.3750851378328934No Hit
CCTGCGGAAGGATCATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC10421920.35954542525113115No Hit
CCTGCGGAAGGATCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG8858090.3055949130258908No Hit
CCTGCGGAAGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT8403870.28992479436660645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGCGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG8059670.27805025154038643Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAG7512380.25916931445914887No Hit
TTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAA5448910.18798174070662035No Hit
TGGAATGAGTACAATGTAAATACCTTCTGTAGGCACCATCAATAGATCGG5176220.1785742186750052No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGAT5009770.1728318663989322No Hit
AGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGA4920240.1697431743035473No Hit
AATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGG4790120.16525416932810347No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGCACTGTAGGCACCATCAATAGATC4789720.16524036974318052No Hit
ACCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4746290.1637420798101685No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCACTGTAGGCACCATCAA4676720.16134198700244004No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCTGTAGGCACCATCAATA4671870.16117466703524896No Hit
AAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCG4559200.15728766895206997No Hit
ATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGA4517600.15585251212008058No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA4446430.15339722097266023No Hit
AACACGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA4432850.15292872506452523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCCTTGTCTATGTTCCTTGGACTGTAGGCACCATCAATAGATCGGAAG4016320.1385588722946082No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA3944570.13608357174904953No Hit
GTTCCTTGTCTATGTTCCTTGGAACTGTAGGCACCATCAATAGATCGGAA3719800.12832923999120677No Hit
TATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGA3482860.12015505586208247No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATCTGTAGGCACCATCA3444480.11883098568872302No Hit
ATACAGTGAAACTGCGAATGGCTCATTACTGTAGGCACCATCAATAGATC3217770.11100972594400382No Hit
ACATTGCGCCCCTTGGTATTCCAGGGGGCACTGTAGGCACCATCAATAGA3174110.10950350124966107No Hit
ACCTGCGGAAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3015790.10404162553714755No Hit
ATTTGTATACGACTTAGATGTACACTGTAGGCACCATCAATAGATCGGAA3003740.10362591304134293No Hit
AGCGGAGAATTTGGACAAACTTGGTCCTGTAGGCACCATCAATAGATCGG2929340.10105919024566956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)