FastQCFastQC Report
Wed 27 Sept 2023
SRR10315313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102665050
Total Bases10.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA36502333.555477740477407Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC11289881.0996809527682498No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA9041350.8806648416379284No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT8943240.8711085223257574No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC7943310.7737112094135248No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG4808540.46837166104725997No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4777070.4653063530383514No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4412870.4298317684547955No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA4383340.4269554244604176No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGG4314560.42025596831638423No Hit
CTACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGG4188630.4079898660741898No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC4045400.3940386723622109No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC4031950.39272858679755185No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA3828540.3729156124698717No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCA3790810.3692405545996422No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA3785560.3687291829108348No Hit
TGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGC3678470.3582981745004751No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC3531090.3439427536440103No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3465430.3375471983893253No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA3425930.3336997352068693No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2982060.29046496349049655No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2954960.2878253115349381No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACC2865160.2790784205530509No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA2822400.2749134199028783No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGC2628080.2559858491278191No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG2584790.2517692242881097No Hit
TTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCAC2346140.22852372837689167No Hit
TTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2328510.2268064935438107No Hit
AGAGCTAATACATGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2195920.21389167978781484No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACC2170930.21145755054909143No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGG2092540.2038220407042124No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT2038150.19852423000816735No Hit
ATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGAA1996440.19446150369575624No Hit
CTACATGGTATAACTGTGGTCTGTAGGCACCATCAATAGATCGGAAGAGC1837320.17896255833898683No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA1754520.17089749627550954No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1723460.16787212396039355No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG1630670.15883399462621406No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTT1625630.15834307780495893No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1621420.15793300641260097No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA1545860.15057315025902193No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTCTGTAG1484280.1445750038596387No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACC1481530.1443071424988348No Hit
AGAGTGTTCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGC1453360.1415632681228909No Hit
CACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGA1429790.1392674527504735No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1381080.13452289751965252No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC1376310.13405827981382176No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1304670.12708024785455227No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1292130.12585880004928648No Hit
AACTTTTCGAATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1290570.1257068496046123No Hit
GCGGCGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1267050.12341590443875497Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCAC1263560.12307596402086202No Hit
TCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGA1256590.12239705722638815No Hit
TATCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG1231500.1199531875745446No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGCA1154150.11241897802611503No Hit
ATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGAA1144450.11147415795346129No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1139450.1109871372974542No Hit
CACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAG1138880.1109316169426694No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT1117450.10884424641102303No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA1107890.10791306291673748No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCT1101630.10730331305541661No Hit
TACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGA1074190.10463054369524974No Hit
TTGCTCCACACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1062230.10346559028608081No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1045800.10186524041044152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)