FastQCFastQC Report
Wed 27 Sept 2023
SRR10315314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115912943
Total Bases11.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2430433020.967744732354866Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGAT20136561.7372141090404372No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT11016910.9504469229117926No Hit
AAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG6188040.5338523757437511Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
NTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5615940.484496368968908Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAGA5486620.47333972013806946No Hit
CCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAGA5177610.44668091983481084No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4459460.3847249396471626Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT4373540.3773124801084552No Hit
AACCTGGATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA3843170.33155658898247453Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG3589320.30965653248921476No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3179340.27428688442497745No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA3128670.2699155002905931No Hit
TGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGC3019710.2605153421046345No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2706920.23353043499206122No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2678670.231093261086469No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC2652570.22884157121262982No Hit
TTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCAC2606890.22490068257519785No Hit
CCGTGAAAATCCACAGGAAGGAACTGTAGGCACCATCAATAGATCGGAAG2474280.21346020003995586No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2468300.21294429561675438Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2434940.21006627361708866No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA2427640.20943649062555506No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG2380860.20540070318117967No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA2369000.2043775214990443No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCA2358910.20350704062444522No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2332840.20125793889988627No Hit
AGGATAAATCCATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2210280.19068448637353638No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA2196630.18950687845101127No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC2174540.1876011378643022No Hit
TTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2171450.18733455848843386No Hit
TGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAGAGCA2048380.17671710742431931No Hit
TTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2032910.17538248511212418No Hit
TCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1956300.16877321456672875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1813920.1564898580825439No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC1811000.15623794488593049No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1784780.15397590241497017No Hit
AGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1692710.1460328722738064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGGAAGAG1682780.1451761948620354No Hit
CCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCGGAAG1620830.13983166659826762No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGG1494410.12892520553118902No Hit
CAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1494010.12889069687411872Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1460850.12602992920298814Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGATAAATCCATAGGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1455800.12559425740747518No Hit
GTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAA1380670.11911266889323999No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACC1348570.11634334916334581No Hit
CTACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGG1311740.11316596456359493No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA1293570.11159840881617508No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGCTGAACTCCAGTCAC1282010.11060110862684247No Hit
AGCTGGGACTGAGGACTGCGACGTCTGTAGGCACCATCAATAGATCGGAA1281390.11054762020838346No Hit
TTAAATCAGTTATCGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1277120.11017924029415767No Hit
AAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1276140.11009469408433535Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGC1263080.10896798643098898No Hit
TTGATGATTCATAATAACTTTCTGTAGGCACCATCAATAGATCGGAAGAG1235740.10660931972023176No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA1206200.1040608553955877No Hit
GTAATTCTAGAGCTAATACATGCTTCTGTAGGCACCATCAATAGATCGGA1176270.10147874513030007No Hit
TTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1172560.10115867733597275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)