FastQCFastQC Report
Wed 27 Sept 2023
SRR10315315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22066337
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA12128475.4963676118968No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT6125882.77612002390791No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3872241.7548177570205694No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT3199991.4501681905791615No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2559321.1598300161916315No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC2305351.0447361517228708No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1894010.8583255118418611No Hit
TCTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1581110.7165258103327253No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1486070.6734556804783685No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1437340.6513722689905443No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1348040.6109033864569366No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1314200.5955678099178854No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT1201640.5445579844085586No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG1135520.5145937905326108No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA1090680.4942732452604163No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATAGATC1027010.46541933987503226No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA833360.37766123122292566No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG804280.3644827866083981No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT801390.3631730993685087No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG781680.35424094175666765No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG751260.3404552373146481No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT742280.33638569011250036No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT700480.3174428089265563No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA662580.3002673257459994No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG572720.2595446629859772No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA542330.2457725539132299No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA539300.24439942161673683No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA527330.23897486927712558No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC441550.20010117673812375No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGCTGTAGGCACCATCA427570.19376573465727456No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA394600.1788244238271173No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAGGCTGTAGGCACCAT380010.1722125425710665No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA375180.17002368811824092No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA361630.16388311299696004No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT356280.16145860547674948No Hit
ACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCTGTAGGCACCATCAAT346060.15682711634468377No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA324200.14692062393500108No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT319180.14464566547678484No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGAT304900.13817426970321353No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGCTGTAGGCACCATCAA301270.1365292300212763No Hit
TTTGCTCCACACAATATAGATGGATACGCTGTAGGCACCATCAATAGATC299930.13592197019378432No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC288150.13058352185956373No Hit
TCTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAA283960.12868470195121193No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG275060.12465140906712338No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA274300.12430699304556075No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCTGTAGGCACCATCAATAG274040.12418916651186827No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG273750.1240577446089036No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC269160.12197765311025568No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA258670.11722380565474005No Hit
ACTCTGGTGGAGGCTCGTAGCGGTTCTGCTGTAGGCACCATCAATAGATC254350.11526607247954203No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAG251700.11406514819383028No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT251360.11391106734207858No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA249450.11304549549841461No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG234450.1062478108623103No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG233670.10589433126123289No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCTGTAGGCACCATCAATAGA230220.10433086379492891No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCCTGTAGGCACCATCAATAGA229600.10404989282996992No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG229080.10381423976258497No Hit
AAATTCGGTAAGCGTTGGATTGTTCACCCACTACTGTAGGCACCATCAAT227060.10289881823158958No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA225630.10225077229628098No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA222930.1010271890617822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)