FastQCFastQC Report
Wed 27 Sept 2023
SRR10315316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224590002
Total Bases22.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG17968820.800072124314777No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA10759740.4790836592984224No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG8445900.3760585923143631No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8415270.37469477381277194Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGAT7311960.3255692566403735No Hit
ATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGATCGG6405140.2851925705935921No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA6204720.27626875394034683No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGATC4629420.20612760847653405No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA4500800.20040072843491938No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA3932810.1751106445067844No Hit
AGCTGGGACTGAGGACTGCGACGTCTGTAGGCACCATCAATAGATCGGAA3920200.17454917694866934No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCCTGTAGGCACCATCAATAGA3672700.16352909601024893No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATAGATC3626450.16146978795609968No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATA3550920.1581067709327506No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGCTGTAGGCACCATCAAT3523060.15686628828651064No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA3455390.15385324231841807No Hit
ATACAGTGAAACTGCGAATGGCTCATTACTGTAGGCACCATCAATAGATC3244500.14446324284729292No Hit
AAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGATCGGAA3238280.14418629374249706No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATAGAT3204940.1427018109203276No Hit
ATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCG3184300.14178280295843265No Hit
AAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCG3083400.1372901719819211No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA3066300.13652878457163023No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAG3021560.1345367101426002No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCCTGTAGGCACCATCAATAGATCG3013490.1341773887156384No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAAT2833160.12614809095553595No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG2806160.12494590030770827No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA2789980.12422547643060264No Hit
AAATTCTAGAGCTAATACATGCTTCTGTAGGCACCATCAATAGATCGGAA2776220.12361280445600603No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTCTGTAGGCACCATCAATAGA2704840.12043456858778602No Hit
AAATGTGATTTCTGCCCAGTGCTCTGACTGTAGGCACCATCAATAGATCG2592170.11541787153998065No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG2457610.1094265095558439No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGGCACCATCAATA2401410.10692417198518034No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCTGTAGGCACCATCAATAGATC2390230.1064263760058206No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC2365380.10531991535402364No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGGCACCATCAA2359010.10503628741229541No Hit
AATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGG2298330.10233447524525156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)