FastQCFastQC Report
Wed 27 Sept 2023
SRR10315318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163264569
Total Bases16.3 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA50775703.1100256663771306Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA33811562.0709674001589407No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG20907301.2805779066491763No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAG18795091.1512044600442366No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC14034200.8595986309803691No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC7589970.4648877614101318No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG6757080.4138730185849448No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA6132070.3755909832463405No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA5999000.36744040894751634No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAG5720230.3503656693572014No Hit
TTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGG5167660.3165206040509622No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC4857970.2975520059101127No Hit
ACCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGT4477890.2742720008037996No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGC4325640.2649466462009893No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTA4294730.26305340015322No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT3997520.2448492054635565No Hit
CTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA3952290.24207885545577254No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA3890020.23826480073579223No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGT3571910.21878047526649827No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGG3505830.21473305699291068No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCG3405060.20856086662624274No Hit
GCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGT3312860.20291359112949975No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC3220170.19723630299725348No Hit
ATGCCTGGGCAGGGCGAAGCCAGAGGCTGTAGGCACCATCAATAGATCGG3039380.1861628655020674No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3032520.18574268860502122No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC2983050.18271263742471888No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG2959700.18128244346757194No Hit
ATCCCGGACGAGCCCCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2948800.18061481545331493No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG2635260.1614104037477966No Hit
TTGCGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACC2520460.15437887200130973No Hit
GTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA2459060.15061810502191691No Hit
TCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGC2432380.14898394764390063No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA2360760.14459720283829616No Hit
AAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTGTAGGCAC2310970.14154755156950188No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG2308010.14136625075095136No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC2247080.1376342714015311No Hit
ACCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGG2189700.1341197305338184No Hit
TGCGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCC2179820.13351457780162945No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCG2173700.13313972610922092No Hit
GGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGT2150280.13170524463271635No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAG2068670.12670660956450386No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT1866070.11429730353803831No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCA1842830.11287384711131047No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA1816860.1112831774296357No Hit
TCCCCGGCATCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1783840.10926069329837267No Hit
GCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAG1779170.10897465450694327No Hit
AACCATCCTCTGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1741780.10668450666721203No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1740710.10661896887131708No Hit
TTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC1733320.10616632932770613No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTCTGT1726150.10572716484493337No Hit
AGATCCCCGAATCCGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA1716350.10512691213486743No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTGTAGGCACCATCAATA1686770.10331512895489284No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC1684950.10320365345159487No Hit
ATCCCATCCTCGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1649160.10101150605432341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)