FastQCFastQC Report
Wed 27 Sept 2023
SRR10315319.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72914343
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC12120481.6622902300580285No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC8600251.1795004447890314No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA8016021.099374919966021No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC5331850.7312484458647595No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG5285500.7248916718621465No Hit
ATGCCTGGGCAGGGCGAAGCCAGAGGCTGTAGGCACCATCAATAGATCGG4398710.6032708818345932No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC4349250.5964875799539193No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTA4157370.570171769908151No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3938630.5401721853271036Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT3317130.45493518332874505No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG3055600.4190670688755983No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA3018540.41398439261806147No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2546030.3491809560706047No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAG2492140.3417900919713423No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC2363100.32409261371250375No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA2343600.3214182427728931No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCA2267590.31099368199751865No Hit
GGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGT2192150.3006472951419174No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT2164130.2968044298225385No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCCTGTAGGCACCA2152520.29521215050926264No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1985490.27230444907115187No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT1780760.24422629714979396No Hit
TGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGCTGTAGGC1747310.23963872238415423No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCA1672910.22943496864533225No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC1573540.21580664863153193No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCA1503800.20624200097366305No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC1445860.19829569060232774No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGC1422920.19514953319952427No Hit
TTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCA1366960.1874747743389802No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG1352270.18546008156447355No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAG1301630.1785149459551463No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC1271900.17443755887644766No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGCTGTAGGC1270730.17427709662007104No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG1251870.17169049990617072No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC1217910.1670329800544181No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1201780.16482079527206328No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGC1191550.16341777913297525No Hit
ATGCCTGGGCAGGGCGAAGCCAGAGCTGTAGGCACCATCAATAGATCGGA1169280.16036351037271226No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1152400.1580484651696032No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG1113510.15271480948542593No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAG1070010.1467489050816792No Hit
GGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGG1065850.14617837261456226No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1059720.14533766010893082No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCCTGTAGGCACC1050100.14401830377872293No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCAC1041870.1428895820949796No Hit
CTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA1037610.14230533490509542No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT1018940.13974479616445284No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGG979900.13439056839612476No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA963920.13219895569792078No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGT962150.13195620510494074No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGC932400.12787607508168866No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGTAGGCACCATCA929990.12754555026299833No Hit
GGGCCGCCGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGAA914050.12535942345390125No Hit
GCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGT908600.12461197106308701No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCA903420.12390154842374429No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCA894930.12273716846080614No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACTGTAGGC852930.1169769848985679No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC837310.11483474520232596No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGG810700.11118525747396504No Hit
TCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGC797240.10933925578949534No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGCTGTAGGCACCA779880.10695837991710355No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGG770740.10570485425617838No Hit
GGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAA745110.10218977081093633No Hit
GTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA742410.10181947329622103No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTGTAGGCACCAT739890.10147386228248673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)