FastQCFastQC Report
Wed 27 Sept 2023
SRR10315320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164227985
Total Bases16.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA71132374.331318441251045Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG8740010.5321876171104456No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA7312120.44524202132785107No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT7269510.44264745743546696No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC7155660.4357150214075877No Hit
ATCCCGGACGAGCCCCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC5573620.33938308382703475No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5325820.32429430343433857No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG5191790.3161330877925586No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTGTAGGCACCATCAATA4882670.2973104736077716No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCA4782180.29119154083270277No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG4638250.2824275046667595No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA4576780.278684537230363No Hit
AAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTGTAGGCAC4343520.2644811114256806No Hit
TTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC4193770.2553626898606836No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4039450.24596599659917887No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA4024560.2450593301744523No Hit
ATCCCATCCTCGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3774680.2298438965807198No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG3681250.2241548539976302No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCAC3616570.22021642657309592No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA3612870.2199911300135601No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC3512400.21387341505773208No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG3512220.21386245468456547No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3456240.2104537786297506No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA3371880.2053170170723339No Hit
ACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGG3308960.20148575774098426No Hit
TTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGC3147000.1916238575295191No Hit
AACCATCCTCTGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3123240.19017708827152693No Hit
TCCCCGGCATCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2964280.18049786094617187No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA2831200.17239449171832683No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT2723180.16581704999912164No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA2683910.16342586191994013No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG2551370.1553553738115949No Hit
TACCTGGTTGATCCTGCCAGTAGCATCTGTAGGCACCATCAATAGATCGG2398870.14606950210099698No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA2388360.14542953808999118No Hit
AACCGGGCGGAAACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2336800.14229000008737855No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA2273360.1384270774557698No Hit
GCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAG2258490.13752162885028396No Hit
TGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG2257210.13744368841887697No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC2177900.13261442621974567No Hit
AAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTGTAGGCACC2170010.13213399652927607No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCTGTAGGCA2164750.13181371006896297No Hit
CCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAG2145850.1306628708864692No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAG2045410.12454698265950229No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1984620.12084542107729081No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1895740.11543343237146823No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC1862470.11340759006450699No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC1859910.113251709201693No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG1859100.11320238752244327No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCTGT1810190.11022421056922789No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGCTGTAGGC1787750.10885781738112417No Hit
ATCCCACTCCTGACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1771820.10788782435587942No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT1742630.10611041717402793No Hit
ATCCCACCGCTGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1738880.10588207606639027No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGCTGTAGGCAC1732690.10550516101138305No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG1697290.10334962095528359No Hit
TCCCCGGCACCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1691000.1029666168040727No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCTGTAG1685530.10263354324173192No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT1660690.1011210117447401No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAT1653460.10068077008921471No Hit
TTCCCGGCCAATGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1644160.10011448414227332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)