FastQCFastQC Report
Wed 27 Sept 2023
SRR10315321.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315321.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65084610
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC9449521.4518824035359512No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5508760.8463997863703877Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA3390970.5210094982515836No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA3187950.4898162561010967No Hit
TACCTGGTTGATCCTGCCAGTAGCATCTGTAGGCACCATCAATAGATCGG2646280.4065907439562133No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG2261050.34740163611643365No Hit
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2123180.3262184408879457No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA2076990.31912152504255614No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1936690.29756496966026225No Hit
TCGTTTCCCGGTCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1845070.2834879090463936No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1807540.2777215688931684No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG1653870.2541107644341727No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA1639880.2519612547420965No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1432750.2201365268993699No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT1425260.21898571720718615No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG1402310.21545953797679668No Hit
AACCATCCTCTGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1380530.2121131247463878No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1285530.1975167401325751No Hit
AACCATCCTCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1257370.1931900644407334No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1244400.19119727382556337No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG1230000.18898476921041701No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG1159130.17809586628851273No Hit
TCGTTTCCCGGTCAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1136160.17456661413504668No Hit
CCTGCGGAAGGATCATTAACGGAGCCTGTAGGCACCATCAATAGATCGGA1083800.16652170152052845No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC1075230.1652049539822087No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1060080.16287721475169015No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1048140.16104267967496463No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGA1017820.15638412829085094No Hit
CCTGCGGAAGGATCATTAACGGAGCTGTAGGCACCATCAATAGATCGGAA996120.15305000675274846No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT868930.1335077524471607No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAG865540.13298689198567834No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGA843010.1295252441398973No Hit
AACCATCCTCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT836330.1284988878323155No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT824090.12661825890944112No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT791480.12160785783305761No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC785920.12075358521776501No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC773580.11885759167950763No Hit
TACCTGGTTGATCCTGCCAGTAGCACTGTAGGCACCATCAATAGATCGGA772020.11861790367953347No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT758570.11655136291052524No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT746970.11476906752610179No Hit
ATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG719240.11050845968040679No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA710020.1090918421420978No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAGATCGGAAGA704090.10818072044988825No Hit
AAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT678860.10430422798876723No Hit
CCGTCTCCGCCCCCCGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGC666430.10239440629666523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)