FastQCFastQC Report
Wed 27 Sept 2023
SRR10315322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10315322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180969785
Total Bases18 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA135978337.513869235132263Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA10109570.5586330336857062No Hit
AACCATCCTCTGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3249570.1795642294651563No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA3050960.16858946923101004No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2995530.16552652698349615Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG2767250.15291226654217444No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC2373560.13115780626031026No Hit
AGATGTCCGGGGCTGCACGCGCGCTCTGTAGGCACCATCAATAGATCGGA2301460.1271737157669718No Hit
AGATGTCCGGGGCTGCACGCGCGCTGTAGGCACCATCAATAGATCGGAAG2257810.12476171091212822No Hit
CCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAAG2111830.11669517096458948No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCAC1986580.10977412610618949No Hit
CAACACGGGAAACCTCACCCGGCCCGGACTGTAGGCACCATCAATAGATC1933330.10683164595680986No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG1865970.1031094776401486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)