FastQCFastQC Report
Mon 25 Sept 2023
SRR1032162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48850266
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length29
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTCCAGTGCGGTAACGCGTGGAATTC9590771.963299442422688No Hit
GGGGAGGTGGAGCACGAGCGCACGTGTTA8631261.7668808599732089No Hit
GAATTCTCGGGTGCCAAGGAACTCCAGTC7168311.4674044968352884RNA PCR Primer, Index 1 (100% over 29bp)
GGCGTCCAGTGCGGTAACGCGATGGAATT4517820.9248301739032495No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGT4297090.8796451589434537No Hit
GCGGGGCGCGGGACATGTGGCGTACGGAA4021310.82319101394453No Hit
GGCGCCCTGGAATGGGTTTGGAATTCTCG3691930.7557645643116866No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAG3671990.7516827032221278No Hit
GGGGAGTGAGAGGGAGAGAACGCGGTCTG3502150.7169152364492755No Hit
TGGGGAGTGAGAGGGAGAGAACGCGGTCT3205890.6562686884857495No Hit
AAGCATCGCGAAGGCCCGCTGGAATTCTC2871830.5878842092691983No Hit
CTCGGGGTGCGCGGCTGGGGGTTCCCTCG2808560.5749323862433011No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTT2653100.5431086086614144No Hit
GGGTGGGGTCCGCGCAGTCCGCCCGGAGG2461050.5037945955094697No Hit
GGGGCGCGGGACATGTGGCGTACGGAAGA2394080.49008535593235053No Hit
CGGACCAAGGAGTCTAACACGTGCGCGAG2377370.4866646990212909No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGA2295730.46995240517216424No Hit
TTCCACCGCGGCGGTGCGCCGCGACCGGC2114340.432820570516443No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGA2069550.42365173610313606No Hit
GGCGCCCTGGAATGGGTTGGAATTCTCGG2051070.4198687474905459No Hit
AGGCGTCCAGTGCGGTAACGCGTGGAATT2046240.4188800118304371No Hit
CGCCGCGGAGCCTCGGTTGGCCTCGGATA1989810.40732838588842074No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGA1841280.37692322903625536No Hit
AAAGCATCGCGAAGGCCCGCGGCGGGTGT1756710.35961114316143133No Hit
AAAGCATCGCGAAGGCCCGCTGGAATTCT1555910.3185059422194344No Hit
GGCGCTGGAGCGTCGGGCCCATACCCGGC1531590.3135274636989694No Hit
GGCGTCCAGTGCGGTAACGCGACCGATCC1518870.3109235884201736No Hit
CTCCATCTAAGGCTAAATACCGGCACGAG1449280.2966780160419188No Hit
CACGGACCAAGGAGTCTAACACGTGCGCG1448230.29646307350711254No Hit
TCCATCTAAGGCTAAATACCGGCACGAGA1390690.28468422259972953No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTAG1389390.2844181032709218No Hit
AGGCGTCCAGTGCGGTAACGCGATGGAAT1388950.2843280321134792No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAA1341700.2746556180471975No Hit
CTCTTAGCGGTGGATCACTCGGCTCGTGC1297280.26556252528901275No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC1271770.2603404452291007No Hit
GGCGCTGCGGGATGAACCGAACGCCGGGT1247290.2553292135604748No Hit
TGAACAGGGAAGAGCCCAGCGCCGAATCC1209490.24759128230744948No Hit
GGGTCTTCCCGGAGTCGGGTTGCTTGGAA1187360.24306111250243756No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTG1172380.23999459900586828No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGCC1146680.2347336245825151No Hit
AAGCATCGCGAAGGCCCGTGGAATTCTCG1143870.23415839741793834No Hit
GCGTCCAGTGCGGTAACGCGTGGAATTCT1128600.23103251884032727No Hit
CGGGTGGGGTCCGCGCAGTCCGCCCGGAG1102230.22563439060905013No Hit
GGCAGCGCCGCGGAGCCTCGGTTGGCCTC1099880.22515332874543612No Hit
TCCACCGCGGCGGTGCGCCGCGACCGGCT1066190.21825674398579528No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGC1030150.21087909736254049No Hit
AACGGGTGGGGTCCGCGCAGTCCGCCCGG1021190.20904492106552705No Hit
CGATGTGGTGACGTCGTGCTCTCCCGGGC1016020.2079865849655762No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTG1011300.2070203670948281No Hit
CGGGGCGCGGGACATGTGGCGTACGGAAG923160.18897747660166272No Hit
GGGTCTTCCCGGAGTCGGGTTGCTTGGGA914020.18710645301296824No Hit
GGCGGCGTCGCGGCGGGTTGGAATTCTCG909140.18610748199405913No Hit
CATCGCGAAGGCCCGCGGCGGGTGTTGAC903150.18488128600978346No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGA901330.1845087189494526No Hit
GGAGCGTCGGGCCCATACCCGGCCGTCGC870030.17810138434046602No Hit
AGCATCGCGAAGGCCCGCTGGAATTCTCG869540.17800107782422311No Hit
GGCAGGGCGCCCTGGAATGGGTTTGGAAT809370.16568384704394445No Hit
CACCGCGGCGGTGCGCCGCGACCGGCTCC801280.16402776599005622No Hit
AGGCGTCCAGTGCGGTAACGCGACCGATC799820.16372889351308753No Hit
GGCCGCCCCCTCGCCCGTCACGCACCGCA779260.15952011397440496No Hit
TCGTGATCGATGTGGTGACGTCGTGCTCT741900.15187225387882228No Hit
GACCAAGGAGTCTAACACGTGCGCGAGTC734670.15039222099629918No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTC722880.1479787233911889No Hit
CTACGGCCATACCACCCTGAACGCGCCCG707920.14491630403813974No Hit
GCGCCGCGGAGCCTCGGTTGGCCTCGGAT703810.14407495754475524No Hit
GGGTCTTCCCGGAGTCGGGTTGCTTTGGA702720.14385182672290872No Hit
TGGCGCTGCGGGATGAACCGAACGCCGGG685080.1402407921381636No Hit
GAGTCTAACACGTGCGCGAGTCGGGGGCT651200.13330531301508164No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT648580.13276898021394604No Hit
GGAAGAGCCCAGCGCCGAATCCCCGCCCC626460.12824085748069416No Hit
CTGGTGGCCCGGCCGTGCCTGAGGTTTCT618180.1265458820633648No Hit
GCCGCCTCTGCGGGCTCCCGGGTGCCCTT615110.12591743103302652No Hit
GTACGACTCTTAGCGGTGGATCACTCGGC614270.12574547700518152No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCG611990.1252787446438879No Hit
CAGCACTCGCCGAATCCCGGGGCCGAGGG610200.12491231879883724No Hit
CCGGGGGAGAGGGTGTAAATCTCGCGCCG604390.1237229701062426No Hit
CTGGTTCCCTCCGAAGTTTCCCTCAGGAT602440.12332379111303099No Hit
CGCGGCGGTGCGCCGCGACCGGCTCCGGG569250.11652955994139315No Hit
CTCGGGGTGCGCGGCTGGGGGTTTGGAAT559850.11460531248693713No Hit
GTCAGAATCCCGCCCAGGCGGAACGATAC558250.1142777810053276No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTG556290.1138765549403559No Hit
AGCCCAGCGCCGAATCCCCGCCCCGCTGG545330.11163296429133059No Hit
AACCGAACGCCGGGTTAAGGCGCCCGATG541320.11081208851554666No Hit
TAGCTGGTTCCCTCCGAAGTTTCCCTCAG538690.110273708642651No Hit
GGGAGTGAGAGGGAGAGAACGCGGTCTGA524650.1073996198915273No Hit
GGCAGGGCGCCCTGGAATGGGTTCGCCCC517930.10602398766876725No Hit
GGGTGTTGACGCGATGTGATTTCTGCCCA501230.1026053778294677No Hit
AGCCTCTGGCATGTTGGAACAATGTAGGT496120.10155932416007725No Hit
GCCTCTGGCATGTTGGAACAATGTAGGTA495530.10143854692623373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)