FastQCFastQC Report
Mon 25 Sept 2023
SRR1032163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49250248
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length44
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC6936261.4083705730781295No Hit
GGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCG4011760.8145664565993657No Hit
GTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGA3869610.7857036577765051No Hit
TGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGAT3359810.6821914886601179No Hit
TGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGC2857570.5802143371948096No Hit
TTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGA2738430.5560235960639224No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGG2038970.41400197619309453No Hit
TTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAATT2004260.4069542959458803No Hit
TGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGTG1956450.3972467306154479No Hit
ATTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAAT1894870.38474324027769363No Hit
TGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGAC1887550.38325695334569687No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGATG1816480.3688265691575807No Hit
GGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCA1792970.3640529891341867No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT1680260.3411678251853676No Hit
GGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT1680170.3411495511657119No Hit
AAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCC1641430.3332836009272481No Hit
TTTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAAT1614430.32780139503053873No Hit
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATG1528890.31043295457111203No Hit
TGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGG1431810.29072137870249914No Hit
ATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGG1315400.267084949501168No Hit
TAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGC1308460.26567581954105085No Hit
CGGCCGCCCCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAAC1245580.25290837114160314No Hit
GTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGT1233250.25040483044877254No Hit
CCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGAT1218290.24736728229266985No Hit
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGC1175730.23872570144215316No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGTGG1174580.23849220007988592No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTG1136450.2307501070857552No Hit
AAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGAT1131190.22968209215921107No Hit
TAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCC1123110.22804149128345508No Hit
AACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTG1113130.22601510554830098No Hit
TTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGT1059950.21521719037841192No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTT1052850.21377557327224017No Hit
AAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATG1045140.2122100989217354No Hit
AAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGC1029230.20897965833593365No Hit
GCGGCCGCCCCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAA1011430.20536546333736228No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTGGAATT1003120.20367816218915283No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGATGGA961200.1951665299228544No Hit
GAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGC960530.19503048999875086No Hit
CGGCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCC939440.1907482780594323No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCTGGAATTCTCG934680.1897817854643087No Hit
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGATGGAAT927980.18842138622327342No Hit
TCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGAT888500.18040518293430727No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATC878480.17837067541263954No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGTGGAA875260.17771687159829125No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAG860800.1747808457736091Illumina Paired End PCR Primer 2 (100% over 23bp)
TTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG850610.1727118206592584No Hit
TGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCG850240.1726366941340072No Hit
TGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAATTC849680.1725229891228162No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGTG846060.17178796744333147No Hit
ATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT844950.17156258786757786No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGG828150.16815143753184755No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGAT822450.16699408295365334No Hit
AGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCG819320.16635855315896073No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAAGGCGCC805420.16353623234546963No Hit
CCGGGCGTCGGCGTCGGGGAGAGCCCGTCCTCCCCGCGTGGCGT801630.16276669307330188No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCG794120.1612418276553653No Hit
TTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGG789280.16025909148721446No Hit
AAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGT788650.16013117334962454No Hit
CAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGTG787370.15987127618118796No Hit
ATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTG777150.15779615972695205No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACC763110.15494541266066314No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGA762620.15484592077587103No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGG759020.1541149599896431No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGTGGAAT756320.15356673939997217No Hit
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTGGAATTCTC744330.15113223389250752No Hit
TCCTGGTGGCCCGGCCGTGCCTGAGGTTTCTCCCCGAGCTGGAA738380.14992411814860304No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT731740.1485759015873382No Hit
ACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGA728190.14785509303425234No Hit
CGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGC725070.14722159368618812No Hit
GGCGGCCGCCCCCTCGCCCGTCACGCACCGCACGTTCGTGGGGA672210.1364886528084082No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAAT671660.1363769782438456No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGATGGAA667050.13544094234814819No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGG659780.13396480764929344No Hit
CAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGAT653180.13262471287454228No Hit
CCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGATGGAA652620.13251100786335127No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATG645260.13101659914484085No Hit
GGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC640110.12997091913120926No Hit
GGCCGCCCCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACC636760.1292907195106916No Hit
AGCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTG636660.1292704150444075No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTTGG635150.12896381760351747No Hit
AAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTTGGA633400.12860848944354555No Hit
TTTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTG626820.1272724555620512No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAA620790.1260480962451194No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAA603340.12250496687854241No Hit
GCGCTGCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGATGCC602370.12230801355558657No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGGT602280.12228973953593086No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGTGGAATTC593410.1204887333765304No Hit
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGTGGAATTCTCGG592410.12028568871368932No Hit
TGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC590250.11984711224195257No Hit
AGCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTC586450.11907554252315643No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCT578010.11736184556877763No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT556720.11303902469689087No Hit
AAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCC551020.11188167011869667No Hit
TCGCCGCCTCTGCGGGCTCCCGGGTGCCCTTGCCCTCGCGGTCC534950.10861874238684037No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGT529880.10758930594623604No Hit
CGGGGGCCCTCCGTCCCCCTAAGCGCAGACCCGGCGGCGTCCGC502490.10202791263101862No Hit
ACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGT495340.10057614329170486No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTTGGAATTCTCGG492670.10003401404191914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)