FastQCFastQC Report
Mon 25 Sept 2023
SRR1032164.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032164.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49978313
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length44
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAG6091441.2188166495335688Illumina Paired End PCR Primer 2 (100% over 23bp)
AGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC3007830.6018270364587937No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGG1595810.3193004933960056No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGT1522130.30455809902987324No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGATGGA1494970.2991237419318255No Hit
GTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGA1451730.29047198932064794No Hit
GGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCG1367340.27358666548028543No Hit
TTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGA1251160.2503405827243509No Hit
TGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGAT1181560.23641454244363952No Hit
AGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCTGGA1098450.21978532968889927No Hit
TGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGAC973730.19483050578357858No Hit
TGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGC973230.19473046239075736No Hit
ACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAA968760.1938360744589358No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGATGGAA965470.1931777889341723No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCTGGAATTCTCG952360.19055465117440037No Hit
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATG907410.1815607501597743No Hit
TCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGAT878720.17582026027969372No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTGGAATT862710.17261687084155883No Hit
GGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT862170.1725088239773119No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATC812820.16263454110585926No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTG797370.15954320026768412No Hit
TGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGTG774820.15503124325144788No Hit
CCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGAT773100.15468709398014294No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGTG755610.1511875760992573No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGTGGAAT748950.14985499810687888No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATC738750.14781411289332635No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCTGGA729400.14594330144756987No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATG728910.14584525892260508No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGA717520.1435662704341381No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGATGGAAT678000.13565884066555028No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGTGGAA676230.13530468705496324No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAAT665170.13309172720575824No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGG662690.13259551197736505No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGTGGAATTC627830.12562048662987085No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGG601520.12035620329961917No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG600310.12011409828899186No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAA596930.11943780495352055No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGGAATT578120.11567417251558691No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTTGG561180.11228470236680457No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGGAATTCTCGG549340.10991567482479851No Hit
AAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTTGGA548480.10974360018914603No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCT545490.10914534070007526No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT544110.10886922093588873No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAA539700.10798683821120574No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGATG529520.10594995473336606No Hit
TACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTTGGA528440.10573386100487225No Hit
AACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTG524350.10491550605159482No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTTGGAATTCTCGG514610.1029666607594378No Hit
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTGGAATTCTC513930.10283060174520095No Hit
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGATGGAAT513060.10265652624169208No Hit
GCGGCCGCCCCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAA512200.1024844516060396No Hit
AAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGAT506630.1013699682100114No Hit
CGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGC504280.10089976426375176No Hit
GCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTGGAATTC499910.10002538501049446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)