FastQCFastQC Report
Mon 25 Sept 2023
SRR1032172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104171446
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length29-43
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA5332170.5118648348223946Illumina Paired End PCR Primer 2 (100% over 22bp)
ATGTGGCGTACGGAAGACCCGTGGAATTCTCGGGTGCCAAGGA2765070.26543454143854356RNA PCR Primer, Index 1 (100% over 22bp)
ATGTGGCGTACGGAAGACCCGCTGGAATTCTCGGGTGCCAAGG2351730.22575572196626703Illumina Small RNA Adapter 2 (100% over 21bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2257590.21671869659945012No Hit
ACGTGCGCGAGTCGGGGGCTTGGAATTCTCGGGTGCCAAGGAG2023950.19429028565082987RNA PCR Primer, Index 1 (95% over 22bp)
AGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCTGGAATTCTC1601700.1537561454220382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)