FastQCFastQC Report
Tue 27 Jun 2023
SRR1032181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120322
Total Bases42.5 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15410.13754974016398858No Hit
GCAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT14070.12558889319320696No Hit
TCAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT13980.12478555272502014No Hit
ATAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT13800.12317887178864648No Hit
TCAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT13390.11951920965579538No Hit
ACAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT13060.11657362793911037No Hit
GCAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT12900.11514546710677824No Hit
CTAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT12640.11282470575423852No Hit
GTAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT12490.11148580497392713No Hit
ACAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT12440.11103950471382336No Hit
ATAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT12270.10952208382947046No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGG11970.10684428226884769No Hit
GTAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT11610.10363092039610042No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGG11600.10354166034407963No Hit
TTAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT11590.10345240029205889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)