FastQCFastQC Report
Tue 27 Jun 2023
SRR1032182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933594
Total Bases35.4 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT15830.16955978723085197No Hit
TTAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT14080.1508150223758936No Hit
TCAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT14000.14995811883966692No Hit
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGA13640.1461020529266469Illumina Paired End PCR Primer 2 (100% over 38bp)
GTAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT13520.1448166976223069No Hit
GCAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT13290.14235309995565523No Hit
GCAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT13260.14203176112957022No Hit
ATAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT12980.1390325987527769No Hit
CTAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT12860.13774724344843692No Hit
ACAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT12450.13335561282527522No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGG12300.13174891869485023No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGG10990.11771712328913853No Hit
CCAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGGT10720.11482507385437353No Hit
ACAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT10600.11353971855003352No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGG10370.11107612088338185No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGG10350.11086189499932518No Hit
ATAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT10260.10989787852107018No Hit
GTAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT10180.10904097498484351No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGG9780.1047564573037102No Hit
TTAAAAAAAAAAAAAAAAAAAAAAATCGGAAGGGGGGT9730.10422089259356851No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGG9640.1032568761153135No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAATGGGAAGGGGGG9410.10079327844866184No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)