FastQCFastQC Report
Thu 24 Aug 2023
SRR10346270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67862131
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length70
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG24844193.6609799359233213No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG18125712.670960922226271No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG12178161.7945442945197225Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA9556791.4082655317735306No Hit
GCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG7952891.1719186949787945No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC6972871.0275053107306638No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC6849271.0092919127458582No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGC6645030.9791955987942672No Hit
CCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA6357120.9367698753816027No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA5733330.8448496850179963No Hit
GCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG5157240.7599584516436715No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA4952820.7298356133260832No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC4733570.697527461965496No Hit
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC4408930.6496892943134958Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG4369840.6439290861644177No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA4181550.6161831257553642No Hit
CCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG4158800.6128307406084845No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC3298780.4861002670252132No Hit
GTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC3170000.4671235567300414No Hit
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA3093660.45587427839541317Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG2817730.4152138988974573No Hit
GGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2561760.377494776873423Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2425930.35747919557669067Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT2415240.3559039429516294No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA2257540.3326656511862264No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC2226230.32805188507858674No Hit
GAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA1935310.2851826153234121No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCG1859820.27405859093932666No Hit
GGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1604590.23644851353105903Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1576320.23228271449359586No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCG1420100.20926251195972612No Hit
GGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1292100.19040074058387585Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCC1172070.17271340919135003No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA1161630.17117499596350724No Hit
GGGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA1122730.16544278575631527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCGG1080500.1592198747781734No Hit
GTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC1064140.15680910462419756No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATCG1034250.15240458629275877No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGC1010830.14895347156133365No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGATC1008510.1486116019551464No Hit
CGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC982640.14479946113098038Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC980200.14443990861412825Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATCGGAAGAGCA970530.1430149607297183No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA958820.14128940336400575No Hit
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA946300.1394444863513054Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT937620.13816542247988056No Hit
GAGGAAGAGCCGACATCGAAGGATCAAAAAAAAAAAAAAAGATCGGAAGA935980.1379237560341275No Hit
GGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC882880.1300990680649271Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG880700.12977782852118216Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA873900.12877579691684013Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCGCGAGGGGGGCCCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCAC868590.12799332811992006No Hit
GGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG865990.12761019838884813No Hit
GGGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT839460.12370080155602539Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC838880.12361533415447858Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG816950.12038378223047551Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCC812140.11967499222799237No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA804680.1185757046149936No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT780160.11496249653580728Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGATC756050.11140970506805924No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA751090.11067881142724505No Hit
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA738180.1087764249548839Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCGG735370.10836235013014844No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATCG727650.10722474954404247No Hit
GAGGAAGAGCCGACATCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG727560.10721148736104383No Hit
GGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG714900.10534594028590112Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAAAGAT714760.10532531022345878No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAA697810.10282759909204736No Hit
GGGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT691040.10182998821537154Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGCGAGGGGGGCCCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACA679910.10018989825120582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)