FastQCFastQC Report
Thu 24 Aug 2023
SRR10346271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61518622
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length70
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG21582413.5082726657954075No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG19800133.2185587642063895Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG16186942.6312260375403076No Hit
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC7205461.171264857005412Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA6517551.0594434316165273No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC5833940.9483209815720515No Hit
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA5133250.8344221364386218Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC4632730.7530614063494465No Hit
GGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT4471600.7268693372228006Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA4259040.692317197872215No Hit
GGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG4025150.6542978157085508No Hit
GGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT3588830.5833729500638035Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG2916000.47400281495251956No Hit
GGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA2854920.4640741140138022Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC2689690.43721558002388283No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA2601750.42292072146869614No Hit
GCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG2577530.4189837022032125No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGC2412310.39212679373735No Hit
GGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA2373550.3858262624933309Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGG2365160.3844624478097055No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2287320.37180936855185087No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA2163080.35161385767060904No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG2113630.3435756412099088No Hit
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA2047720.33286181215177413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCG1856920.30184681314870804No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1775150.28855490293654495No Hit
CCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGG1723330.2801314372743915No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1714660.27872210791717666Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA1713020.2784555219718673No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1623750.2639444687171309No Hit
GGGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1599210.2599554326818309Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1573470.2557713337597191Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG1530180.24873444011798573No Hit
CCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG1497790.2434693676981256No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCG1490650.2423087435215958No Hit
GGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1487300.24176419296257967Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1475800.23989484029730054Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA1422380.23121129078606475Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1399180.2274400749743712Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC1299250.21119621307512382No Hit
GGGGAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1264070.2054776194434264Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT1224460.19903891865458234Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1193900.19407131713711012No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1191940.1937527144219843No Hit
GGGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA1129380.18358343592286577Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGC1108640.18021209902913626No Hit
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1090000.1771821221873273Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1080060.1755663512749034Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT1041460.16929182841579254Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1005400.16343018866709988Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC984990.16011249406724357Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC969030.15751815767264749Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCC943510.15336982027978455Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG931540.15142406798383748No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA926040.150530029752617No Hit
GTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC917800.1491905979298431No Hit
GGGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC916930.1490491773369046Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAGATCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC915880.14887849731094432Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGC913100.1484266016231638No Hit
GGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC902390.14668566535836905Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC891420.14490246546809843Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGAGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC879810.14301523203819486Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG868360.14115400699319955Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA861600.14005515273082678Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG748400.12165422040825297Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC741640.12055536614588018Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT725730.11796915737156792No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA678840.11034707506939931No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCGG657720.10691396826151275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)