FastQCFastQC Report
Tue 25 Jul 2023
SRR10346272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64600808
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length70
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG36597375.6651566958729065No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG25983574.022174149896082No Hit
GCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG15486252.3972223381478446No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG15162822.3471564008920756Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG13476012.0860435677522795No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC8753861.355069738446615No Hit
GGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG8448251.307762280620391No Hit
GCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA6707121.0382408839220711No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC6435150.9961407913040345No Hit
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC4842330.7495773117884222Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC4687720.7256441746053703No Hit
CCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA4451290.6890455611638789No Hit
GCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGG4292110.6644050024885138No Hit
GCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG3822960.5917820718279561No Hit
CCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGG3319060.5137799514829597No Hit
GGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT3219310.4983389681441755Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGC3205000.496123825571965No Hit
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA3164870.48991182896659746Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC3105640.48074321299510686No Hit
CGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG3032980.4694956756578029No Hit
GTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC2974980.4605174597816176No Hit
GGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2762990.4277020807541603Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG2098150.32478695932100415No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA2097960.32475754792416833No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG2060820.3190083938269007No Hit
CCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG2047250.3169078009055243No Hit
GGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA2030110.31425458331728606Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1915670.29653963461261973No Hit
GGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGC1894230.29322079067494017No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA1838920.2846589782592193No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1791450.277310772955038No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCG1713360.2652226888555326No Hit
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1486230.23006368589073997Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1436170.22231455680863932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410990.21841677274377125No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA1333510.2064231147077913No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCG1268820.19640930806933563No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1191700.18447137689051815No Hit
GGGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA1183970.18327479742977829Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1165320.18038783663510835No Hit
CCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC1109220.17170373472728082No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1107690.17146689558434006No Hit
CGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1020180.15792062538908183Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC988860.15307238881594173No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC986100.15264514957769568Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA946570.14652603106759904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC925290.14323195462199173Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG859240.13300762430092206Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA833010.12894730356932996Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT824090.12756651588630286No Hit
CCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG821930.12723215474332766No Hit
GGGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT820730.1270463985527859Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG817300.12651544544148738Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT805280.12465478759956068Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT804550.1245417859169811No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGC762940.11810069000994539No Hit
GGGGAGAGATCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC754890.11685457556506104Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA738540.11432364746892949No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA734460.1136920764210875No Hit
GGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC732510.11339022261145713Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATCGGAAGAGCA730440.11306979318277258No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT720440.11152182492825786Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC707160.10946612308626233Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG679690.10521385429111041No Hit
GGGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC668400.10346619813176332Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)