FastQCFastQC Report
Tue 18 Jul 2023
SRR10346277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53582598
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG10878892.0303028233158833Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC5715221.066618680938166Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4214590.7865594721629586No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3925450.7325979229301274No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3826190.7140732519166016No Hit
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA3780500.705546229766612Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2171410.4052453746270384Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC2110260.3938330873766143Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1905620.3556415834857429Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCCGCGAGGGGGGCCCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCAC1779400.33208542818323217No Hit
GGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1728920.3226644590842721Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1714550.3199826182373613Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1328750.24798163015537245Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1307440.2440045926851102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1217510.2272211586306435Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1193860.22280741221245Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA1175740.2194257172823162No Hit
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1067560.19923632668949723Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC1055010.19689414835764402Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1036310.19340420932930502Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1035200.193197052520671Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA958720.178923761778031No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA954400.17811752987415802No Hit
CCCGCGAGGGGGGCCCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACC798890.14909504761228637No Hit
GGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC785150.14653078225135707Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCC755900.14107192040221714Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA733010.13680001107822357Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA717460.13389794947979194No Hit
GGGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC716660.133748647275371Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC696810.1300440863281769Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC692370.12921545909364082Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC676030.1261659615683435Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC661940.12353637649298005Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGCGAGGGGGGCCCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACA651830.12164956988461068No Hit
CAGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC648720.12106915756492434Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG647440.12083027403785088Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCGCGAGGGGGGCCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA637810.11903304875213404No Hit
GGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC561570.10480454867082033Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA560500.10460485697240735No Hit
GGGGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC559690.10445368849043117Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA548520.10236905646120406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAACAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC536240.10007726762334293Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)