FastQCFastQC Report
Tue 18 Jul 2023
SRR10346278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55936925
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG12816212.291189585412498Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC6471471.1569227303789045Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA4206310.7519737633057234Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3853590.688917025739259No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3576550.6393898127220973No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3489640.6238526697704602No Hit
GGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2749520.49153935437101703Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCCGCGAGGGGGGCCCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCAC2702290.4830959156228198No Hit
CGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC2365360.42286200036916577Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2363130.4224633370532971Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1780520.31830852339487736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1780330.3182745565652742Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1525850.27278045763151976Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1479060.264415678909772Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1424910.2547351324728701Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA1350570.24144516345866346No Hit
GGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC1337370.23908536266518046Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1297670.2319880830059929Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1233650.22054304915760028Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1191010.21292017750350062Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1118290.19991982040485778Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA1085170.19399886568666405No Hit
GGGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA933330.16685400565011396Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC913100.16323743216131384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC844920.1510487035173993Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCC840540.15026567870865265Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC812940.14533154977682453Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC761300.13609972303625914Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC756600.1352594909355493Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC739730.13224359401236302Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCGCGAGGGGGGCCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA732420.13093676493657813No Hit
GGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC711750.12724153142132857Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA694470.12415233765531444No Hit
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA688030.12300104090455453Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG677920.12119364802409141Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAACAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC650190.11623627862990323Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC649630.1161361658689676Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAGAGATCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC643340.11501168503631545Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGCGAGGGGGGCCCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACC642470.11485615271129043No Hit
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA615580.11004895245850571Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA595390.10643952988120102Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC568370.10160908916605624Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)