FastQCFastQC Report
Tue 18 Jul 2023
SRR10346279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45707374
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG24884795.444370967362946Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC9396472.0557886348929166Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA5782941.2652094167562546Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT4871991.0659089712745258Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC3260330.7133050347630997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT3238690.7085705689414579Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2907210.636048354035828No Hit
GGGGAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC2847480.6229804407490135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2809710.6147170038690036No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2764610.604849887022606No Hit
GGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC2580870.5646506841543774Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA2523110.552013773532472Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA2081860.4554757400851775Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA2071320.45316976643637413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1953300.42734898749597816Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1775240.38839247251439124Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1576670.3449487165900189Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1545620.3381555019984303Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC1432850.31348333422086333Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCC1273090.2785305495782803Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1222900.2675498268616351Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1107390.2422781934486107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1078760.23601443390731655Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA1073010.2347564312051705Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1071560.23443919574115107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA1062860.23253578295703445Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1005830.220058583982532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC996310.21797576907393543Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC974710.21325005457543894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA953170.20853746706166057Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG945350.20682658338674192Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC937980.20521415209720867Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA915960.20039654870568588Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC911660.19945578146755927Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAACAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC896330.19610183687209856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC814870.1782797672865652Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCC808270.17683579896758014Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGAGAGATCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC803220.17573094442047799Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGAGAGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC795020.17393692317567838Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG778790.17038607380944704Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA737020.16124750461490087Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAAAAAAAAAAAAAAAGAACGGAAGAGCACACGTCTGAACTCCAG733310.1604358193931684Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT729760.15965913946401736Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG705030.15424863392939617Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA656420.1436135884769928Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA647280.14161391113827715Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT645200.14115884233471826Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT641740.14040185288264428Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGAGACAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA626270.13701727865617483Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA591390.12938612487341758No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG574620.1257171326447238Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAAAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC549480.12021692604786265Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA537150.11751933068830425Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGAGATCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT509090.11138027750183155Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGAGAGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG497910.10893428268270236Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC492830.10782286464324116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAAAGAGCGGAAGAGCACACGTCTGAACTCCAG466580.10207980882909616Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGGGAGAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC461730.1010187108977208Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA459650.10056364209416187Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC457160.10001887222836298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)