FastQCFastQC Report
Tue 25 Jul 2023
SRR10346280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42243000
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG20363814.820635371540847Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC9661022.287010865705561Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA5685471.3458963615273536Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT4141700.9804464645029944Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC3342290.7912056435385744Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3204400.7585635489903653No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2998150.7097388916506877No Hit
GGGGAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC2952390.6989063276755911Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC2709070.6413062519233956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2551150.6039225433799682Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2446250.5790900267499941No Hit
GGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA2232320.5284473167151954Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA2198850.5205241105035153Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG2089010.49452216935350235Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1999260.4732760457353881Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1459080.3454016049996449Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1404750.3325403025353313Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1340130.317243093530289Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCC1320420.31257723173070096Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1232090.291667258480695Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1122450.2657126624529508Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1083350.25645669104940466Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1014170.24008001325663422Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC996100.23580238145965013Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA982520.23258764765760007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC914840.21656605828184552Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC900520.213176147527401Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC874760.20707809577918238Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC862940.20427999905309754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC850260.201278318301257Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAGAGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC844490.19991241152380276Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG824700.19522761167530717Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG818990.19387590843453353Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA803430.19019245792202258Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGAGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA802100.1898776128589352Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA792580.18762398503894137Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC791680.18741093198873185Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCC751650.1779348057666359Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGAGAAAGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA742290.17571905404445706Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720170.17048268352153018No Hit
CGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA713240.16884217503491702Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGACAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA698980.16546646781715316Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA640920.1517221788225268Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAGATCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC630880.14934545368463414Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG630090.14915844045167245Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAACAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC620140.14680302061880077Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT614440.14545368463414055Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAGAAAAAAAAAAAAAAAGAACGGAAGAGCACACGTCTGAACTCCAG612740.14505125109485595Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT611130.1446701228605923Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG609860.14436948133418553Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG561690.13296640863575032Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA505690.1197097744004924Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGAGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC503370.11916057098217456Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAGATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACG501530.11872499585730181Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GGGGAGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT488580.11565939919039842Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGG461070.10914707762232796Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GAGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC457290.10825225481144805Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCC451010.10676561797220842Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGAGAAAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC448530.10617853845607556Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGG438570.1038207513670904Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431350.1021115924531875No Hit
GGGGAGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC428640.10147006604644555Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC425480.10072201311459887Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA423570.10026986719693205No Hit
GGGGAGAAAAAAAAAAAAAAAGAGCGGAAGAGCACACGTCTGAACTCCAG423110.10016097341571384Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)